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Ferulic Acid Modulates Fluoride-Induced Oxidative Hepatotoxicity in Male Wistar Rats

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Abstract

The present study is aimed to evaluate the protective effect of ferulic acid (FA) on fluoride-induced oxidative hepatotoxicity in male Wistar rats. Fluoride (25 mg/L) was given orally to induce hepatotoxicity for 12 weeks. Hepatic damage were assessed using status of pathophysiological markers like serum marker enzymes like aspartate transaminase, alanine transaminase, alkaline phosphatase, acid phosphatase, gamma glutamyl transferase, lactate dehydrogenase, bilirubin, lipid profile, total protein content levels, and histopathological studies. Treatment with FA significantly reduced the degree of histological abberations and rescued lipid peroxidation, as observed from reduced levels of lipid hydroperoxides, nitric oxide, restored levels of enzymic and non-enzymic antioxidants, and total protein content, with a concomitant decline in the levels of marker enzymes and lipid profile in fluoride-induced rats. These results suggest that ferulic acid has the ability to protect fluoride-induced hepatic damage.

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Abbreviations

FA:

Ferulic acid

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

CAT:

Catalase

GSH:

Reduced glutathione

GPx:

Gultathione peroxidase

TBARS:

Thiobarbituric acid reactive substances

LOOH:

Lipid hydroperoxides

Vitamin C:

Ascorbic acid

NO:

Nitric oxide

AST:

Aspartate transaminase

ALT:

Alanine transaminase

LDH:

Lactate dehydrogenase

GGT:

Gamma glutamyl transferase

ALP:

Alkaline phosphatase

ACP:

Acid phosphatase

TG:

Triglycerides

LDL-C:

Low-density lipoprotein

HDL-C:

High-density lipoprotein

VLDL-C:

Very low-density lipoprotein

DMRT:

Ducan’s multiple range test

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Acknowledgments

The authors thank the management of Karunya University for providing Karunya Research Grant to carry out this work successfully and thank Dr. Pauline Das, English Department, for language proof reading and Mr. Prakash for his assistance in histological and biochemical works of this study.

Conflicts of Interest

The authors have declared no conflicts of interest.

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Authors and Affiliations

  1. Department of Biotechnology, School of Biotechnology and Health Sciences, Karunya University, Karunya Nagar, 641 114, Coimbatore, Tamil Nadu, India

    Lakshmikanthan Panneerselvam, Kavitha Subbiah, Angelin Arumugam & J. Giftson Senapathy

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  1. Lakshmikanthan Panneerselvam
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  2. Kavitha Subbiah
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  3. Angelin Arumugam
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  4. J. Giftson Senapathy
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Correspondence to Lakshmikanthan Panneerselvam.

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Panneerselvam, L., Subbiah, K., Arumugam, A. et al. Ferulic Acid Modulates Fluoride-Induced Oxidative Hepatotoxicity in Male Wistar Rats. Biol Trace Elem Res 151, 85–91 (2013). https://doi.org/10.1007/s12011-012-9534-2

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  • DOI: https://doi.org/10.1007/s12011-012-9534-2

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