Abstract
Both fluoride and lead can cross the blood-brain barrier and produce toxic effects on the central neural system, resulting in low learning and memory abilities, especially in children. In order to identify the proteomic pattern in the cortex of young animals, from the beginning of fertilization to the age of postnatal day 56, pregnant female mice and pups were administrated with 150 mg sodium fluoride/L and/or 300 mg lead acetate/L in their drinking water. Two-dimensional electrophoresis (2-DE) combined with mass spectrometry (MS) was applied to identify differently expressed protein spots. Results showed that there were eight proteins in the cortex that significantly changed, whose biological functions were involved in (1) energy metabolism (Ndufs1, Atp5h, Atp6v1b2), (2) cytoskeleton (Spna2, Tuba1a, Tubb2a), (3) glycation repair (Hdhd2), and (4) cell stress response (Hspa8). Based on the previous and current studies, ATPase, Spna2, and Hspa8 were shared by fluoride and lead both as common target molecules.
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Acknowledgments
This research was supported by the China National Natural Science Foundation (31101869, 31201965, and 31172376), Shanxi Province Natural Science Foundation (2012021027-5 and 2011021030-3), and Program for the Outstanding Innovative Teams of Higher Learning Institutions of Shanxi, Talent Introduction Start-up Projects of Shanxi Agricultural University (XB2010003).
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Ruiyan Niu and Yuliang Zhang contributed equally to this work as co-first authors.
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Niu, R., Zhang, Y., Liu, S. et al. Proteome Alterations in Cortex of Mice Exposed to Fluoride and Lead. Biol Trace Elem Res 164, 99–105 (2015). https://doi.org/10.1007/s12011-014-0205-3
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DOI: https://doi.org/10.1007/s12011-014-0205-3