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Indigofera oblongifolia Ameliorates Lead Acetate-Induced Testicular Oxidative Damage and Apoptosis in a Rat Model

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Abstract

Lead (Pb) enhances the production of reactive oxygen species and depletes the antioxidant molecules that cause tissue damage. In the current study, we investigated the protective effect of Indigofera oblongifolia (hasr in Arabic) against lead acetate-induced reproductive toxicity in rats. Exposure of rats to lead acetate (PbAc; 20 mg/kg body weight; intraperitoneal injection) induced a significant change in both of body weight loss and the relative testis weight. Furthermore, a significant increase in lipid peroxidation and nitric oxide and a marked depletion of glutathione were evident in the testis of the PbAc group compared to the control group. Also, PbAc significantly reduced the activity of antioxidant enzymes. Pre-administration of I. oblongifolia leaves extract (IOLE; 100 mg/kg body weight) to the PbAc-treated rats restored most of the parameters mentioned above to near-normal levels. Additionally, pretreatment of animals with IOLE accompanied with a significant decrease in the toxic effects of PbAc as shown by caspase-3 and Bax expressions and prevented the histological injury in the testis. On the basis of the above results, I. oblongifolia appeared to be a promising agent for protection against lead-induced oxidative damage and apoptosis in the testis of rat.

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References

  1. Abdel-Moneim AE, Dkhil MA, Al-Quraishy S (2011) The redox status in rats treated with flaxseed oil and lead-induced hepatotoxicity. Biol Trace Elem Res 143(1):457–467

    Article  CAS  PubMed  Google Scholar 

  2. Elgawish RAR, Abdelrazek HMA (2014) Effects of lead acetate on testicular function and caspase-3 expression with respect to the protective effect of cinnamon in albino rats. Toxicology Reports 1 (0):795–801

  3. Ayinde OC, Ogunnowo S, Ogedegbe RA (2012) Influence of vitamin C and vitamin E on testicular zinc content and testicular toxicity in lead exposed albino rats. BMC Pharmacol Toxicol 13:17

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Abdel Moneim AE (2012) Flaxseed oil as a neuroprotective agent on lead acetate-induced monoamineric alterations and neurotoxicity in rats. Biol Trace Elem Res 148(3):363–370

    Article  CAS  PubMed  Google Scholar 

  5. Mabrouk A, Cheikh HB (2014) Thymoquinone supplementation ameliorates lead-induced testis function impairment in adult rats. Toxicol Ind Health. doi:0748233714548474

  6. Newman DJ, Cragg GM (2012) Natural products as sources of new drugs over the 30 years from 1981 to 2010. J Nat Prod 75(3):311–335

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Ali NA, Julich WD, Kusnick C, Lindequist U (2001) Screening of Yemeni medicinal plants for antibacterial and cytotoxic activities. J Ethnopharmacol 74(2):173–179

    Article  CAS  PubMed  Google Scholar 

  8. Shahjahan M, Vani G, Devi CS (2005) Protective effect of Indigofera oblongifolia in CCl4-induced hepatotoxicity. J Med Food 8(2):261–265

    Article  CAS  PubMed  Google Scholar 

  9. Lubbad MY, Al-Quraishy S, Dkhil MA (2015) Antimalarial and antioxidant activities of Indigofera oblongifolia on Plasmodium chabaudi-induced spleen tissue injury in mice. Parasitol Res 114(9):3431–3438

    Article  PubMed  Google Scholar 

  10. Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95(2):351–358

    Article  CAS  PubMed  Google Scholar 

  11. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR (1982) Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem 126(1):131–138

    Article  CAS  PubMed  Google Scholar 

  12. Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82(1):70–77

    Article  CAS  PubMed  Google Scholar 

  13. Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70(1):158–169

    CAS  PubMed  Google Scholar 

  14. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29(9):e45

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Sternberger LA (1979) Immunocytochemistry. Wiley medical publication, 2d edn. Wiley, New York

  16. Ponce-Canchihuaman JC, Perez-Mendez O, Hernandez-Munoz R, Torres-Duran PV, Juarez-Oropeza MA (2010) Protective effects of Spirulina maxima on hyperlipidemia and oxidative-stress induced by lead acetate in the liver and kidney. Lipids Health Dis 9:35

    Article  PubMed  PubMed Central  Google Scholar 

  17. Graca A, Ramalho-Santos J, de Lourdes PM (2004) Effect of lead chloride on spermatogenesis and sperm parameters in mice. Asian J Androl 6(3):237–241

    CAS  PubMed  Google Scholar 

  18. Acharya UR, Acharya S, Mishra M (2003) Lead acetate induced cytotoxicity in male germinal cells of Swiss mice. Ind Health 41(3):291–294

    Article  CAS  PubMed  Google Scholar 

  19. Abdel Moneim AE, Dkhil MA, Al-Quraishy S (2011) The protective effect of flaxseed oil on lead acetate-induced renal toxicity in rats. J Hazard Mater 194:250–255

    Article  CAS  PubMed  Google Scholar 

  20. Abdel Moneim AE (2015) The neuroprotective effect of berberine in mercury-induced neurotoxicity in rats. Metab Brain Dis. doi:10.1007/s11011-015-9652-6

    PubMed  Google Scholar 

  21. Abdel Moneim AE, Dkhil MA, Al-Quraishy S (2011) The redox status in rats treated with flaxseed oil and lead-induced hepatotoxicity. Biol Trace Elem Res 143(1):457–467

    Article  CAS  PubMed  Google Scholar 

  22. Bazrgar M, Goudarzi I, Lashkarbolouki T, Elahdadi Salmani M (2015) Melatonin ameliorates oxidative damage induced by maternal lead exposure in rat pups. Physiol Behav 151:178–188

    Article  CAS  PubMed  Google Scholar 

  23. Elgawish RAR, Abdelrazek HMA (2014) Effects of lead acetate on testicular function and caspase-3 expression with respect to the protective effect of cinnamon in albino rats. Toxicology Reports 1:795–801

    Article  CAS  Google Scholar 

  24. Wang C, Zhang Y, Liang J, Shan G, Wang Y, Shi Q (2006) Impacts of ascorbic acid and thiamine supplementation at different concentrations on lead toxicity in testis. Clin Chim Acta 370(1–2):82–88

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding the work through the research group project no. RGP-198.

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Authors and Affiliations

  1. Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia

    Mohamed A. Dkhil & Saleh Al-Quraishy

  2. Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt

    Mohamed A. Dkhil & Ahmed E. Abdel Moneim

Authors
  1. Mohamed A. Dkhil
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  2. Ahmed E. Abdel Moneim
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  3. Saleh Al-Quraishy
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Corresponding author

Correspondence to Ahmed E. Abdel Moneim.

Ethics declarations

In all experiments, we followed the European Community Directive (86/609/EEC) and national rules on animal care that were carried out in accordance with the NIH Guidelines for the Care and Use of Laboratory Animals 8th edition. This was approved by the committee of the Zoology and Entomology department, Helwan University, Egypt.

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Dkhil, M.A., Moneim, A.E.A. & Al-Quraishy, S. Indigofera oblongifolia Ameliorates Lead Acetate-Induced Testicular Oxidative Damage and Apoptosis in a Rat Model. Biol Trace Elem Res 173, 354–361 (2016). https://doi.org/10.1007/s12011-016-0689-0

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  • DOI: https://doi.org/10.1007/s12011-016-0689-0

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