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Effect of Lead on Antioxidant Ability and Immune Responses of Crucian Carp

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Abstract

The aim of this study was to explore the effects of lead exposure on the antioxidant and immune responses of Crucian carp. Three hundred sixty healthy Crucian carp were randomly grouped into four groups and exposed to different doses of lead (0, 0.05, 0.5, and 1 mg/L). Fish were sampled at 30 and 60 days, respectively, and antioxidant capability, immune parameters, ALAD activity, and immune-related genes were assessed. The results showed that T-AOC and GSH activities of the liver were significantly increased in 60 days (P < 0.05); the activities of SOD, CAT, T-AOC, and GSH were significantly increased (P < 0.05) compared to the control in the kidney in 60 days. With an increase in Pb dose, the activity and expression of lysozyme and the content of immunoglobulin M were significantly decreased compared to the control. Compared with the control group, the activity of ALAD in the lead-exposed group decreased significantly (P < 0.05). The expression of the HSP70, tumor necrosis factor-α (TNF-α), interleukins (IL-10), and immunoglobulin M genes was all enhanced in lead-exposed group, whereas lysozyme gene expression was decreased. The results indicated that lead induced oxidative stress and had immunotoxic effects on Crucian carp.

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Funding

This study was funded by the National Natural Sciences Foundational of China (no. 30972191), and the 948 Program from the Ministry of Agriculture of China (no. 2014Z34).

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Authors and Affiliations

  1. College of Animal Science and Technology, Jilin Agriculture University, Changchun, 130118, China

    Jing Dai, Linbo Zhang, Xiyao Guo & Yuehong Li

  2. Freshwater Fisheries Research Institute of Jilin Province, Changchun, 130000, China

    Xiaoyan Du

  3. Health monitoring and inspection center of Jilin Province, Changchun, 130062, China

    Peijun Zhang

  4. China-Japan union Hospital of Jilin University, Jilin University, Changchun, 130000, China

    Wei Li

Authors
  1. Jing Dai
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  2. Linbo Zhang
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  3. Xiaoyan Du
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  4. Peijun Zhang
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  5. Wei Li
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  6. Xiyao Guo
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Correspondence to Yuehong Li.

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Dai, J., Zhang, L., Du, X. et al. Effect of Lead on Antioxidant Ability and Immune Responses of Crucian Carp. Biol Trace Elem Res 186, 546–553 (2018). https://doi.org/10.1007/s12011-018-1316-z

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  • DOI: https://doi.org/10.1007/s12011-018-1316-z

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