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Streptozotocin (STZ)-Induced Diabetes Affects Tissue Trace Element Content in Rats in a Dose-Dependent Manner

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Abstract

The objective of the present study was investigation of tissue trace element distribution in a streptozotocin model of DM1 in rats. DM1 was modeled in 2-month-old male Wistar rats (n = 30) using intraperitoneal injection of 45 mg/kg b.w. (STZ1) and 55 mg/kg b.w. streptozotocin (STZ2), whereas control animals were injected with physiological saline. The rats were subjected to oral glucose tolerance test (OGTT) and HbA1c level assessment at day 14. At day 30, blood serum, liver, kidney, and heart samples were collected for tissue trace element assessment using inductively coupled plasma mass spectrometry (ICP-MS). STZ-treated rats were characterized by lack of significant weight gain and elevated HbA1c and blood glucose levels. ICP-MS analysis demonstrated a dose-dependent accumulation of Cu, Mn, Mo, and Se levels in the liver. Correspondingly, the dose-dependent increase in renal Cu, Mn, V, and Zn levels was significant, whereas the observed trend for kidney V and Mo accumulation was nearly significant. The patterns of trace element content in the myocardium of STZ-exposed rats were quite different from those observed for liver and kidney. Only cardiac Zn content was characterized by a significant decrease. Serum Co, Cr, Cu, Se, V, and Mo levels were characterized by a significant decrease in response to STZ-induced diabetes. Generally, the obtained data demonstrate that diabetes is associated with altered copper, manganese, molybdenum, chromium, and vanadium handling. In turn, only altered Zn status may provide a link to diabetic cardiotoxicity. However, the particular mechanisms of both impaired metal handling in STZ diabetes and their potential anti-diabetic activity require further investigation.

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Authors and Affiliations

  1. Institute of General Pathology and Pathophysiology, 125315, Moscow, Russia

    Mikhail Yu. Karganov, Irina B. Alchinova, Yulia S. Medvedeva, Marina A. Lebedeva, Margarita V. Polyakova & Nadezhda N. Khlebnikova

  2. Yaroslavl State University, 150003, Yaroslavl, Russia

    Alexey A. Tinkov, Olga P. Ajsuvakova & Anatoly V. Skalny

  3. IM Sechenov First Moscow State Medical University, 119146, Moscow, Russia

    Alexey A. Tinkov, Olga P. Ajsuvakova, Margarita G. Skalnaya, Tatiana I. Burtseva, Svetlana V. Notova & Anatoly V. Skalny

  4. Orenburg State University, Pobedy Ave. 13, 460352, Orenburg, Russia

    Tatiana I. Burtseva

  5. Federal Research Centre of Biological Systems and Agro-technologies of the Russian Academy of Sciences, 460000, Orenburg, Russia

    Svetlana V. Notova

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  1. Mikhail Yu. Karganov
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Correspondence to Mikhail Yu. Karganov.

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The investigation protocol was approved by the Local Ethics Committee (Institute of General Pathology and Pathophysiology, Russian Academy of Sciences, Moscow, Russia). All animal studies were performed in accordance with the ethical standards laid down in the US guidelines (NIH Publication no. 85-23, amended in 1985).

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Karganov, M.Y., Alchinova, I.B., Tinkov, A.A. et al. Streptozotocin (STZ)-Induced Diabetes Affects Tissue Trace Element Content in Rats in a Dose-Dependent Manner. Biol Trace Elem Res 198, 567–574 (2020). https://doi.org/10.1007/s12011-020-02090-2

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