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Hydrogen-Rich Water Alleviates the Nickel-Induced Toxic Responses (Inflammatory Responses, Oxidative Stress, DNA Damage) and Ameliorates Cocoon Production in Earthworm

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Abstract

In recent years, studies investigating the protective effect of hydrogen-rich water (HRW) against different diseases and the toxicity of some substances have attracted increasing attention. Here, we assessed the effects of hydrogen-rich water on different nickel-induced toxic responses (reactive oxygen species (ROS), tumor necrosis factor-alpha (TNF-α), and 8-hydroxy-2′-deoxyguanosine (8-OHdG) of stress responses, histopathological changes) and cocoon production in earthworm model. Earthworms were randomly divided into two main groups: water (W) group including control (CW: ultrapure water), 10 (10W), 200 (200W), and 500 (500W), and hydrogen-rich ultrapure water (HRW) group including control (CHRW: hydrogen-rich ultrapure water), 10 (10HRW), 200 (200HRW), and 500 (500HRW) mg of nickel chloride kg−1 soil for 14 days. We found that cocoon production was less affected by the nickel exposure of earthworms in the 500HRW group compared to the 500W group. The ROS levels in 200HRW and 500HRW groups were less than that of 200W and 500W, respectively. The epithelial degeneration, epithelial necrosis, and necrosis in muscle fibers in tissues of earthworm were less damaged in 200HRW and 500HRW groups compared to 200W and 500W, respectively. HRW groups significantly reduced the expression of 8-OHdG induced by nickel exposure and inflammatory cytokine response including TNF-α. The study showed that hydrogen-rich water could alleviate the toxic effects of nickel-induced oxidative and inflammatory damages in earthworms. The HRW treatment known for its cheap and eco-friendly propertıes without any negative effects on the ecosystem can be used as a green method for alleviating the toxification effects of heavy metals in contaminated soil and increasing cocoon production of earthworms.

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Authors and Affiliations

  1. Department of Organic Agriculture Management, College of Applied Sciences, Igdir University, Igdir, Turkey

    Mine Köktürk

  2. Department of Pathology, Faculty of Veterinary, Ataturk University, Erzurum, Turkey

    Serkan Yıldırım

  3. Tuzluca Vocational School, Laboratory and Veterinary Health Programs, Igdir University, 76000 , Igdir, Turkey

    Gizem Eser

  4. Department of Food Engineering, Faculty of Engineering, Igdir University, 76000, Igdir, Turkey

    Menekşe Bulut

  5. Research Center for Redox Applications in Foods (RCRAF), Igdir University, 76000 , Igdir, Turkey

    Menekşe Bulut & Duried Alwazeer

  6. Innovative Food Technologies Development, Application, and Research Center, Igdir University, 76000, Igdir, Turkey

    Menekşe Bulut & Duried Alwazeer

  7. Department of Nutrition and Dietetics, Faculty of Health Sciences, Igdir University, 76000, Igdir, Turkey

    Duried Alwazeer

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  1. Mine Köktürk
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  2. Serkan Yıldırım
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Contributions

Mine Köktürk: conducting experiments and proofing manuscript, Serkan Yildirim: conducting experiments, Gizem Eser: conducting experiments, Menekşe Bulut: conducting experiments, Duried Alwazeer: supervisor and drafting the manuscript.

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Correspondence to Duried Alwazeer.

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Highlights

• Cocoon production of earthworms exposed to nickel was ameliorated in the presence of hydrogen-rich water

• Hydrogen-rich water attenuated nickel-induced oxidative stress in earthworms by decreasing ROS levels

• Hydrogen-rich water reduced DNA damage (8-OHdG) caused by high nickel exposure

• Hydrogen-rich water reduced inflammatory responses (TNF-α) produced by nickel exposure

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Köktürk, M., Yıldırım, S., Eser, G. et al. Hydrogen-Rich Water Alleviates the Nickel-Induced Toxic Responses (Inflammatory Responses, Oxidative Stress, DNA Damage) and Ameliorates Cocoon Production in Earthworm. Biol Trace Elem Res 200, 3442–3452 (2022). https://doi.org/10.1007/s12011-021-02908-7

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