Abstract
This study evaluates antioxidative and cardioprotective effects of total flavonoids extracted from Dracocephalum moldavica L. (DML). The total flavonoids showed remarkable scavenging effects against 1,1-diphenyl-2-picrylhydrazyl, hydroxyl and superoxide anion radicals in vitro. Compared with the ischemia/reperfusion (I/R) group as demonstrated by the use of improved Langendorff retrograde perfusion technology, the total flavonoids (5 μg/mL) pretreatment improved the heart rate and coronary flow, rised left ventricular developed pressure and decreased creatine kinase, lactate dehydrogenase levels in coronary flow. The infarct size/ischemic area at risk of DML-treated hearts was smaller than that of I/R group; the superoxide dismutase activity and glutathione/glutathione disulfide ratio increased and malondialdehyde content reduced obviously (P < 0.01) in total flavonoids treatment groups. In conclusion, the total flavonoids possess obvious protective effects on myocardial I/R injury, which may be related to the improvement of myocardial oxidative stress states.
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Abbreviations
- AAR:
-
The area at risk
- AN:
-
The area of necrosis
- CF:
-
Coronary flow
- CK:
-
Creatine kinase
- DML:
-
Dracocephalum moldavica L.
- DPPH:
-
1,1-Diphenyl-2-picrylhydrazyl
- +dp/dt max :
-
Maximum rise velocity
- −dp/dt max :
-
Maximum down velocity
- GSH/GSSG:
-
Glutathione/glutathione disulfide
- HR:
-
Heart rate
- I/R:
-
Ischemia/reperfusion
- LVDP:
-
Left ventricular developed pressure
- LDH:
-
Lactate dehydrogenase
- LV:
-
The left ventricle
- LVEDP:
-
Left ventricular end-diastolic pressure
- LVSP:
-
Left ventricular systolic pressure
- MDA:
-
Malondialdehyde
- SOD:
-
Superoxide dismutase
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Acknowledgments
This work was supported by the Xinjiang production and Construction Corps funds for Distinguished Young Scientists (2011CD006) to Qiusheng Zheng.
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The authors declare no conflict of interest.
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Jiangtao Jiang and Xuan Yuan have contributed equally to this work.
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Jiang, J., Yuan, X., Wang, T. et al. Antioxidative and Cardioprotective Effects of Total Flavonoids Extracted from Dracocephalum moldavica L. Against Acute Ischemia/Reperfusion-Induced Myocardial Injury in Isolated Rat Heart. Cardiovasc Toxicol 14, 74–82 (2014). https://doi.org/10.1007/s12012-013-9221-3
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DOI: https://doi.org/10.1007/s12012-013-9221-3