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Androgen receptor coregulator long noncoding RNA CTBP1-AS is associated with polycystic ovary syndrome in Kashmiri women

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Abstract

Objective

Polycystic ovary syndrome (PCOS) is one of the most common reproductive, endocrine, and metabolic disorder in premenopausal women. Even though the pathophysiology of PCOS is complex and obscure, the disorder is prominently considered as the syndrome of hyperandrogenism. C-Terminal binding protein 1 antisense (CTBP1-AS) acts as a novel androgen receptor regulating long noncoding RNA (lncRNA). Therefore, the present study was aimed to establish the possible association of androgen receptor regulating long noncoding RNA CTBP1-AS with PCOS.

Methods

A total of 178 subjects including 105 PCOS cases and 73 age-matched healthy controls were recruited for the study. The anthropometric, hormonal, and biochemical parameters of all subjects were analyzed. Total RNA was isolated from peripheral venous blood and expression analysis was done by quantitative real-time PCR. The correlation analysis was performed to evaluate the association between and various clinical parameters and lncRNA CTBP1-AS expression.

Results and conclusion

The mean expression level of CTBP1-AS was found to be significantly higher in the PCOS women than in the healthy controls (-lnCTBP1-AS, 4.23 ± 1.68 versus 1.24 ± 0.29, P < 0.001). Furthermore, subjects with higher expression level of CTBP1-AS had significantly higher risk of PCOS compared to subjects with low levels of CTBP1-AS expression (actual OR = 11.36, 95% CI = 5.59–23.08, P < 0.001). The area under receiver operator characteristic (ROC) curve was 0.987 (SE 0.006, 95% CI 0.976–0.99). However, lncRNA CTBP1-AS was found to have no association with different clinical characteristics of PCOS. In conclusion, androgen receptor coregulating lncRNA CTBP1-AS is associated with PCOS women and high expression of CTBP1-AS is a risk factor for PCOS in Kashmiri women

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Data availability

The data and materials will be provided by authors on reasonable request.

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Acknowledgements

We thank all the study subjects. We are also thankful to Suhail Murtaza, technical assistant, who assisted in sampling of PCOS cases and control women. We are thankful to co-scholars for their help during the course of experimental studies.

Funding

This work was supported by the Indian Council of Medical Research under grant No. RBMH/FW/2019/3.

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Authors and Affiliations

  1. Department of Biochemistry, University of Kashmir, Hazratbal, Srinagar, 190006, India

    Mudasar Nabi, Sairish Ashraf & Shajrul Amin

  2. Division of Biotechnology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Shuhama, Kashmir, 190006, India

    Syed Mudasir Andrabi

  3. Department of Biotechnology, University of Kashmir, Hazratbal, Srinagar, 190006, India

    Shayaq Ul Abeer Rasool

  4. Cutis Institute of Dermatology, Hyderpora, Srinagar, 190014, India

    Imran Majid

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Contributions

M.N.: major work done, manuscript writing, project development, recruitment of participants, sample collection, data collection. S.M.A: primer designing and qPCR validation. S.U.A.R.: data collection, Data analysis, manuscript revision. S.A.: recruitment of participants, manuscript revision, data collection. I.M.: diagnosis and recruitment of cases. S.A.: conception and design, manuscript writing, project development and management. All authors read and approved the final draft.

Corresponding author

Correspondence to Shajrul Amin.

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The authors declare no competing interests.

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All participants were recruited after written informed consent was obtained from them.

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This study was approved by the ethical committee Government Medical College Srinagar under ethical approval no. 94/ETH/GMC/ICMR.

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Nabi, M., Andrabi, S.M., Rasool, S.U.A. et al. Androgen receptor coregulator long noncoding RNA CTBP1-AS is associated with polycystic ovary syndrome in Kashmiri women. Endocrine 75, 614–622 (2022). https://doi.org/10.1007/s12020-021-02894-9

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