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Molecular Marker-Assisted Genotyping of Mungbean Yellow Mosaic India Virus Resistant Germplasms of Mungbean and Urdbean

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Abstract

Mungbean Yellow Mosaic India Virus (MYMIV) belonging to the genus begomovirus causes the yellow mosaic disease in a number of economically important edible grain legumes including mungbean (Vigna radiata), urdbean (Vigna mungo) and soybean (Glycine max). The disease is severe, critical, open spread and inflicts heavy yield losses annually. The objective of this study is to develop molecular markers linked to MYMIV-resistance to facilitate genotyping of urdbean and mungbean germplasms for MYMIV-reaction. Resistance-linked molecular markers were successfully developed from consensus motifs of other resistance (R) gene or R gene homologue sequences. Applying linked marker-assisted genotyping, plant breeders can carry out repeated genotyping throughout the growing season in absence of any disease incidence. Two MYMIV-resistance marker loci, YR4 and CYR1, were identified and of these two CYR1 is completely linked with MYMIV-resistant germplasms and co-segregating with MYMIV-resistant F2, F3 progenies of urdbean. The present study demonstrated that these two markers could be efficiently employed together in a multiplex-PCR-reaction for genotyping both V. mungo and V. radiata germplasms from field grown plants and also directly from the seed stock. This method of genotyping would save time and labour during the introgression of MYMIV-resistance through molecular breeding, as methods of phenotyping against begomoviruses are tedious, labour and time intensive.

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Acknowledgements

We are thankful to the Director, Bose Institute for providing us all the lab and field facilities and for a research fellowship to S.M, we also thank the Department of Biotechnology, India, for the financial assistance (Sanction no. BT/01/COE/06/03).

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Authors and Affiliations

  1. Division of Plant Biology, Bose Institute, P 1/12 CIT Scheme VIIM, Kolkata, 700054, India

    Soumitra Maiti, Jolly Basak, Sabyasachi Kundagrami, Anirban Kundu & Amita Pal

  2. Centre for Biotechnology, Visva-Bharati University, Santiniketan, 731235, West Bengal, India

    Jolly Basak

  3. Department of Genetics & Plant Breeding, Institute of Agricultural Science, Calcutta University, Kolkata, 700019, West Bengal, India

    Sabyasachi Kundagrami

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  1. Soumitra Maiti
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Correspondence to Amita Pal.

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Supplementary Figure 1a, b

PCR amplification of a polymorphic fragment (456 bp; a) with RGASF1/RGASR1 primers from MYMIV-tolerant lines, VM1, VM4 and VM6 and another fragment amplified with combination of RGA22F2/RGA24R2 primers (1236 bp; b); both are absent in the susceptible cultivar T-9 and in bulked susceptible F2 progenies (S bulk). Two representative phenotyped bulked resistant F2 samples (R bulk) amplified with the same primer pairs like the parental lines. M=Molecular weight marker. (JPEG 292 kb)

Supplementary Figure 2a, b

Germplasm screening for MYMIV-reaction of V. mungo employing markers, YR4 (a) and CYR1 (b). Sources of germplasms are given in Table 1. (JPEG 312 kb)

Supplementary Figure 3a, b

Germplasm screening of V. radiata employing markers, YR4 (a) and CYR1 (b). Sources of germplasms are given in Table 1. (JPEG 366 kb)

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Maiti, S., Basak, J., Kundagrami, S. et al. Molecular Marker-Assisted Genotyping of Mungbean Yellow Mosaic India Virus Resistant Germplasms of Mungbean and Urdbean. Mol Biotechnol 47, 95–104 (2011). https://doi.org/10.1007/s12033-010-9314-1

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