Abstract
Nanotechnology is an emerging field in science and technology, which can be applied to synthesize new materials at the nanoscale level. The present investigation aimed at comparing the synthesis, characterization and in vitro anticancer efficacy of synthesized silver and gold nanoparticles using leaves extract of Bauhinia tomentosa Linn. Silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) were synthesized using aqueous extract of leaves with solution of silver nitrate (AgNO3, 1 mM) and chloroauric acid (HAuCl4⋅3H2O, 1 mM), respectively. The synthesized nanoparticles were characterized using UV–visible spectrophotometry, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, high-resolution transmission electron microscopy, energy-dispersive analysis of X-rays, X-ray diffraction, thermogravimetric analysis and cyclic voltammetry, which confirmed the reduction of Ag+ ions to Ag0 and Au3+ ions to Au0. The in vitro anticancer efficacy of AgNPs, AuNPs and aqueous extract of leaves confirmed by MTT assay exhibited IC50 concentrations of 28.125, 46.875 and 50 μg ml−1 for lung A-549 cells, 103.125, 34.375 and 53.125 μg ml−1 for HEp-2 cells and 62.5, 23.4 and 13.26 μg ml−1 for MCF-7 cells, respectively. The concentrations indicate that both silver and gold nanoparticles as well as aqueous extract of leaves exhibited high anticancer efficacy.
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Faraday M 1857 Philos. Trans. R. Soc. Lond. 147 145
Schoen G and Simon U 1995 Colloid Polym. Sci. 273 202
Montemerlo M S, Love J C, Opiteck G J, Goldhaber Gorden D and Eleenbogen J V 1996 MTRE corporation technical report
Dirix Y, Bastioan C, Caseri W and Smith P 1999 Adv. Mater. 11 223
Wohctjen H and Snow A W 1998 Anal. Chem. 70 2856
Storhoff J J, Elghanian R, Mucic R C, Mirkin C A and Letisinger R L 1998 J. Am. Chem. Soc. 120 1959
Mirkin C A, Letisinger R L, Mucic R C and Storhoff J J 1996 Nature 382 607
Elghanian R, Storhoff J J, Mucic R C, Letisinger R L and Mirkin C A 1997 Science 277 1078
Retchkiman-Schabes P S, Canizal G, Becerra-Herrera R, Zorrilla C, Liu H B and Ascencio J A 2006 Opt. Mater. 29 95
Gu H, Ho P L, Tong E, Wang L and Xu B 2003 Nano Lett. 3 1261
Ahmad Z, Pandey R, Sharma S and Khuller G K 2005 Ind. J. Chest Dis. Allied Sci. 48 171
Gong P, Li H, He X, Wang K, Hu J and Tan W 2007 Nanotechnology 18 604
Frattini A, Pellegri N, Nicastro D and De Sanctis O 2005 Mater. Chem. Phys. 94 148
Everts M, Saini V, Leddon J L, Kok R J, Stoff-Khalili M, Preuss M A et al 2006, Nano Lett. 6 587
Paciotti G F, Kingston D G I and Tamarkin L 2006 Drug Dev. Res. 67 47
Narayanan K B and Sakthivel N 2010 Adv. Colloid Interf. Sci. 156 1
Sharma N C, Sahi S V, Nath S, Parsons J G, Gardea-Torresdey J L and Pal T 2007 Environ. Sci. Technol. 41 5137
Mohanpuria P, Rana N K and Yadav S K 2008 J. Nanopart. Res. 10 507
Kumar V and Yadav S K 2009 J. Chem. Technol. Biotechnol. 84 151
Shankar S S, Ahmad A and Sastry M 2003 Biotechnol. Prog. 19 1627
Li S, Shen Y, Xie A, Yu X, Qiu L, Zhang L et al 2007, Extract. Green Chem. 9 852
Shankar S S, Rai A, Ahmad A and Sastry M 2004 J. Colloid Interf. Sci. 275 496
Nadagouda M N and Varma R S 2008 Green Chem. 10 859
Ankamwar B, Damle C, Ahmad A and Sastry M 2005 J. Nanosci. Nanotechnol. 5 1665
Song J Y and Kim B S 2009 Bioprocess Biosyst. Eng. 32 79
Rita Anusha Grace N, Santhi Swaroop M and Vijaya Lakshmi N 2014 Int. J. Univers. Pharm. Life Sci. 3 296
Mannangatti V, Ayyasamy B, Rangasamy M, Emin B and Natesan S K 2010 J. Global Pharma Technol. 2 71
Rhama S and Madhavan S 2012 J. Drug Deliv. Therap. 2 76
Mukundan D, Mohankumar R and Vasanthakumari R 2014 Int. J. Innovat. Res. Sci. Eng. 2 375
Mukundan D, Mohankumar R and Vasanthakumari R 2015 Mater. Today: Proc. 2 4309
Saini J, Kashyap D, Batra B, Kumar S, Kumar R and Malik D K 2013 Indian J. Appl. Res. 3 209
Mosmann T 1983 J. Immunol. Methods 65 55
Singh A K, Talat M, Singh D P and Srivastava O N 2010 J. Nanopart. Res. 12 1667
Dinesh S, Karthikeyan S and Arumugam P 2012 Arch. Appl. Sci. Res. 4 178
Anuradha J, Abbasi T and Abbasi S A 2015 J. Adv. Res. 6 711
Mittal A K, Kaler A and Banerjee U C 2012 Nano Biomed. Eng. 4 118
Ismail E H, Khalil M M H, Al Seif F A and El-Magdoub F 2014 Prog. Nanotechnol. Nanomater. 3 1
Kirubha R and Alagumuthu G 2013 Asian J. Pharm. Clin. Res. 6 60
Kirubha R and Alagumuthu G 2014 Int. J. Adv. Sci. Techn. Res. 4 891
Niranjana V A, Narendhar C and Anbarasan B 2012 Int. J. Drug Discov. Herb. Res. 2 504
Sathishkumar M, Pavagadhi S, Mahadevan A and Balasubramanian R 2015 Ecotoxicol. Environ. Saf. 114 232
Rosarin F S, Arulmozhi V, Nagarajan S and Mirunalini S 2013 Asian Pac. J. Trop. Med. 6 1
Nachiyar V, Sunkar S, Prakash P and Bavanilatha M 2015 Der. Pharma. Chem. 7 31
Rejeeth C, Nataraj B, Vivek R and Sakthivel M 2014 Med. Aromat. Plants 3 163
Kamala Priya M R and Iyer P R 2015 Appl. Nanosci. 5 443
Ho Y F, Karsani S A, Yong W K and Malek S N A 2013 Evid.-Based Complement. Alternat. Med. 2013 Article ID 857257, 10
Arulvasu C, Babu G, Manikandan R, Srinivasan P, Sellamuthu S, Prabhu D et al 2010, J. Pharm. Res. 3 1485
Yadav S, Seer H and Murthy R R 2012 Int. J. Pharm. Res. Bio-Sci. 1 160
Acknowledgement
We are thankful to the Department of Chemistry, B S Abdur Rahman University, Chennai, The Director, NCNSNT, University of Madras, Chennai, and Royal Bio Research Centre, Chennai, for providing necessary facilities to complete the present study.
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MUKUNDAN, D., MOHANKUMAR, R. & VASANTHAKUMARI, R. Comparative study of synthesized silver and gold nanoparticles using leaves extract of Bauhinia tomentosa Linn and their anticancer efficacy. Bull Mater Sci 40, 335–344 (2017). https://doi.org/10.1007/s12034-017-1376-2
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DOI: https://doi.org/10.1007/s12034-017-1376-2