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Single-nucleotide polymorphisms in peroxisome proliferator-activated receptor γ and their association with plasma levels of resistin and the metabolic syndrome in a South Indian population

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Abstract

Studies on the association of the Pro12Ala and C1431T polymorphisms of PPARγ with diabetes and obesity have revealed extensive population-dependent variations. However, association of these polymorphisms with the metabolic syndrome and its individual components has not been well investigated in the Indian population. The Indian population harbours the maximum number of diabetics in the world who are thus more susceptible to metabolic disorders. We screened a South Indian population (N = 699) for a possible association of these polymorphisms with the metabolic syndrome (MS) and type 2 diabetes. We also investigated the correlation of these two single-nucleotide polymorphisms (SNPs) with plasma resistin levels. The C1431T SNP was associated with higher levels of plasma resistin (P = 0.017). Furthermore, C1431T was associated with resistin in different tertiles. Prevalence of the ‘Pro-C’ haplotype decreased with increasing tertiles of resistin (84.1% to 75.4%, P = 0.037). Plasma resistin levels were not found to be associated with MS and type 2 diabetes. These results point to a likely association of plasma resistin levels with PPARγ polymorphisms in the Indian population.

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Abbreviations

BMI:

body mass index

ELISA:

enzyme-linked immunosorbent assay

EM:

expectation-maximization

HDL:

high-density lipoprotein cholesterol

IDF:

International Diabetes Federation

IGT:

impaired glucose tolerance

IL:

interleukin

IRS:

insulin resistance syndrome

LD:

linkage disequilibrium

MS:

metabolic syndrome

NCEP ATP-III:

National Cholesterol Education Program (NCEP) Adult Treatment Panel III

PCR:

polymerase chain reaction

PPAR:

peroxisome proliferator-activated receptor

PPREs:

PPAR response elements

RFLP:

restriction fragment length polymorphism

SNP:

single-nucleotide polymorphism

T2DM:

type 2 diabetes mellitus

TNF:

tumour necrosis factor

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Authors and Affiliations

  1. Molecular Biology Unit, National Institute of Nutrition, Jamai Osmania, Hyderabad, 500 604, India

    Abdul Haseeb, Mohammad Iliyas, Sudip Ghosh, Madhuri Suragani & Nasreen Z. Ehtesham

  2. L V Prasad Eye Institute, Banjara Hills, Hyderabad, 500 034, India

    Subhabrata Chakrabarti

  3. Mediciti Hospital, Hyderabad, 500 063, India

    Arif A. Farooqui & Sudhir R. Naik

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  1. Abdul Haseeb
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  2. Mohammad Iliyas
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  3. Subhabrata Chakrabarti
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  4. Arif A. Farooqui
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  5. Sudhir R. Naik
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  6. Sudip Ghosh
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  7. Madhuri Suragani
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  8. Nasreen Z. Ehtesham
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Correspondence to Nasreen Z. Ehtesham.

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Haseeb, A., Iliyas, M., Chakrabarti, S. et al. Single-nucleotide polymorphisms in peroxisome proliferator-activated receptor γ and their association with plasma levels of resistin and the metabolic syndrome in a South Indian population. J Biosci 34, 405–414 (2009). https://doi.org/10.1007/s12038-009-0047-x

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  • DOI: https://doi.org/10.1007/s12038-009-0047-x

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