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Integration of advanced technologies for plant variety and cultivar identification

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Abstract

Identification of plant variety and cultivar is pivotal in the agricultural sector due to the abundance of plant varieties and cultivars developed in many crop species. However, plant variety and cultivar identification via basic morphological features is problematic and challenging when differentiating closely related species not only due to their limited differences but also due to technical limitations of the process being time-consuming, labour-intensive and costly, and statistically imprecise information being available due to phenotypic plasticity. Therefore, it is imperative to have rapid and highly efficient techniques to mitigate these limitations. This review provides an overview and summarization of the development and application of molecular markers such as Random Amplified Polymorphic DNA (RAPD), Restriction Fragment Length Polymorphism (RFLP), Simple Sequence Repeats (SSR), Inter-simple sequence repeats (ISSR), Amplified Fragment Length Polymorphism (AFLP), Single nucleotide polymorphism (SNP) and DNA barcoding, High-resolution melting (HRM) and biosensor technology as potential tools in the identification of plant variety and cultivar.

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Acknowledgements

We would like to thank to the Malaysian Agricultural Research and Development Institute (MARDI) for the research funding, PRB 405, MARDI Development Grant. Schematic representations were generated in Adobe Illustrator with the support of Biorender (©BioRender - biorender.com).

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  1. Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Serdang, Malaysia

    Mohammad Malek Faizal Azizi, Han Yih Lau & Norliza Abu-Bakar

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  1. Mohammad Malek Faizal Azizi
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  2. Han Yih Lau
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  3. Norliza Abu-Bakar
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Correspondence to Han Yih Lau.

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Corresponding editor: Manchikatla Venkat Rajam

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Azizi, M.M.F., Lau, H.Y. & Abu-Bakar, N. Integration of advanced technologies for plant variety and cultivar identification. J Biosci 46, 91 (2021). https://doi.org/10.1007/s12038-021-00214-x

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