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Association of ADAM33 gene polymorphisms with adult-onset asthma and its severity in an Indian adult population

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Abstract

ADAM33, a member of the ADAM (a disintegrin and metalloprotease) gene family, is an asthma susceptibility gene originally identified by positional cloning. In the present study, we investigated the possible association of five single-nucleotide polymorphisms (SNPs) in the ADAM33 (rs511898, rs528557, rs44707, rs597980 and rs2787094) with adult-onset asthma in an Indian population. The study included 175 patients with mild intermittent (n = 44), mild persistent (n = 108) or moderate persistent (n = 23) subgroups of asthma, and 253 nonasthmatic control individuals. SNPs were genotyped with the help of restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR) method, and data were analysed using chi-square test and logistic regression model. Bonferroni’s correction for multiple comparisons was applied for each hypothesis. Genotypes and allele frequencies of SNPs rs511898 and rs528557 were significantly associated with adult-onset asthma (P = 0.010-<0.001). A significant association of the homozygous mutant genotype and mutant alleles of SNPs rs2787094, rs44707 and rs597980 with the asthma was also observed (P = 0.020-<0.001). A positive association between asthma and haplotypes AGCCT, GGCCT, AGACT, GCAGT, GGACT, ACCCC and AGACC were also found (P = 0.036-<0.001, OR = 2.07–8.49). Haplotypes AGCGT, GCAGC, ACAGC, ACAGT, GGAGC and GGCGT appear to protect against asthma (P = 0.013-<0.0001, OR = 0.34–0.10). Our data suggest that ADAM33 gene polymorphisms serve as genetic risk factors for asthma in Indian adult population.

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References

  • Aggarwal A. N., Chaudhry K., Chhabra S. K., D’Souza G. A., Gupta D., Jindal S. K. et al. 2006 Prevalence and risk factors for bronchial asthma in Indian adults: a multicentre study. Indian J. Chest Dis. Allied Sci. 48, 13–22.

    PubMed  CAS  Google Scholar 

  • Awasthi S., Tripathi P., Ganesh S. and Husain N. 2011 Association of ADAM33 gene polymorphisms with asthma in Indian children. J. Hum. Genet. 56, 188–195.

    Article  PubMed  CAS  Google Scholar 

  • Bijanzadeh M., Ramachandra N. B., Mahesh P. A., Mysore R. S., Kumar P., Manjunath B. S. and Jayaraj B. S. 2010 Association of IL-4 and ADAM33 gene polymorphisms with asthma in an Indian population. Lung 5, 415–422.

    Article  Google Scholar 

  • Blakey J., Halapi E., Bjornsdottir U. S., Wheatley A., Kristinsson S., Upmanyu R. et al. 2005 Contribution of ADAM33 polymorphisms to the population risk of asthma. Thorax 60, 274–276.

    Article  PubMed  CAS  Google Scholar 

  • Global strategy for asthma management and prevention 2005 Global Initiative for Asthma (Gina), National Heart, Lung and Blood Institute, US Department of Health and Human Services: National Institute of Health (NIH) Publication no. 02–3659.

  • Gunn T. M., Azarani A., Kim P. H., Hyman R. W., Davis R. W. and Barsh G. S. 2002 Identification and preliminary characterization of mouse Adam33. BMC Genet. 3, 2.

    Article  PubMed  Google Scholar 

  • He L. N., Xiong D. H., Liu Y. J., Zhang F., Recker R. R. and Deng H. W. 2007 Association study of the oestrogen signalling pathway genes in relation to age at natural menopause. J. Genet. 86, 269–276.

    Article  PubMed  CAS  Google Scholar 

  • Hirota T., Hasegawa K., Obara K., Matsuda A., Akahoshi M., Nakashima K. et al. 2006 Association between ADAM33 polymorphisms and adult asthma in the Japanese Population. Clin. Exp. Allergy 36, 884–891.

    Article  PubMed  CAS  Google Scholar 

  • Hoffjan S. and Ober C. 2002 Present status on the genetic studies of asthma. Curr. Opin. Immunol. 14, 709–717.

    Article  PubMed  CAS  Google Scholar 

  • Howard T. D., Postma D. S., Jongepier H., Moore W. C., Koppelman G. H., Zheng S. L. et al. 2003 Association of a disintegrin and metalloprotease 33 (ADAM33) gene with asthma in ethnically diverse populations. J. Allergy Clin. Immunol. 112, 717–722.

    Article  PubMed  CAS  Google Scholar 

  • Illig T. and Wjst M. 2002 Genetics of asthma and related phenotypes. Paediatr. Respir. Rev. 3, 47–51.

    Article  PubMed  Google Scholar 

  • Kedda M. A., Duffy D. L., Bradley B., O’Hehir R. E. and Thompson P. J. 2006 ADAM33 haplotypes are associated with asthma in a large Australian population. Eur. J. Hum. Genet. 14, 1027–1036.

    Article  PubMed  CAS  Google Scholar 

  • Lee J. H., Park H. S., Park S. W., Jang A. S., Uh S. T., Rhim T. et al. 2004 ADAM33 polymorphism: association with bronchial hyper-responsiveness in Korean asthmatics. Clin. Exp. Allergy 34, 860–865.

    Article  PubMed  CAS  Google Scholar 

  • Lind D. L., Choudhry S., Ung N., Ziv E., Avila P. C., Salari K. et al. 2003 ADAM33 is not associated with asthma in Puerto Rican or Mexican populations. Am. J. Respir. Crit. Care Med. 168, 1312–1316.

    Article  PubMed  Google Scholar 

  • Newman-Taylor 1995 Environmental determinants of asthma. Lancet 345, 296–299.

    Article  PubMed  CAS  Google Scholar 

  • Noguchi E., Ohtsuki Y., Tokunaga K., Yamaoka-Sageshima M., Ichikawa K., Aoki T. et al. 2006 ADAM33 polymorphisms are associated with asthma susceptibility in a Japanese population. Clin. Exp. Allergy 36, 602–608.

    Article  PubMed  CAS  Google Scholar 

  • Palmer L. J. and Cookson W. O. 2002 Genomic approaches to understanding asthma. Genome Res. 10, 1280–1287.

    Article  Google Scholar 

  • Postma D. S. and Howard T. 2004 ADAM33 gene: confirming a gene without linkage. Clin. Exp. Allergy 34, 1–3.

    Article  PubMed  CAS  Google Scholar 

  • Raby B. A., Silverman E. K., Kwiatkowski D. J., Lange C., Lazarus R. and Weiss S. T. 2004 ADAM33 polymorphisms and phenotype associations in childhood asthma. J. Allergy Clin. Immunol. 113, 1071–1078.

    Article  PubMed  CAS  Google Scholar 

  • Sakagami T., Jinnai N., Nakajima T., Sekigawa T., Hasegawa T., Suzuki E. et al. 2007 ADAM33 polymorphisms are associated with aspirin-intolerant asthma in the Japanese population. J. Hum. Genet. 52, 66–72.

    Article  PubMed  CAS  Google Scholar 

  • Schedel M., Depner M., Schoen C., Weiland S. K., Vogelberg C., Niggemann B. et al. 2006 The role of polymorphisms in ADAM33, a disintegrin and metalloprotease 33, in childhood asthma and lung function in two German populations. Respir. Res. 7, 91.

    Article  PubMed  Google Scholar 

  • Schneider S., Roessli D. and Excoffier L. 2000 Arlequin ver. 2.00. Software for population genetics data analysis, Genetics and Biometry Laboratory, University of Geneva, Switzerland.

    Google Scholar 

  • Shapiro S. D. and Owen C. A. 2002 ADAM-33 surfaces as an asthma gene. N. Engl. J. Med. 347, 936–938.

    Article  PubMed  Google Scholar 

  • Su D., Zhang X., Sui H., Lü F., Jin L. and Zhang J. 2008 Association of ADAM33 gene polymorphisms with adult allergic asthma and rhinitis in a Chinese Han population. BMC Med. Genet. 9, 82–87.

    Article  PubMed  Google Scholar 

  • Thongngarm T., Jameekornrak A., Limwongse C., Sangasapaviliya A., Jirapongsananuruk O., Assawamakin A. et al. 2008 Association between ADAM33 polymorphisms and asthma in a Thai population. Asian Pac. J. Allergy Immunol. 26, 205–211.

    PubMed  CAS  Google Scholar 

  • Upadhyay R., Jain M., Kumar S., Ghoshal U. C. and Mittal B. 2009 Functional polymorphisms of cyclooxygenase-2 (COX-2) gene and risk for esophageal squmaous cell carcinoma. Mutat. Res. 663, 52–59.

    Article  PubMed  CAS  Google Scholar 

  • Van Eerdewegh P., Little R. D., Dupuis J., Del Mastro R. G., Falls K., Simon J. et al. 2002 Association of the ADAM33 gene with asthma and bronchial hyperresponsiveness. Nature 418, 426–430.

    Article  PubMed  Google Scholar 

  • Vercelli D. 2004 Genetics, epigenetic, and the environment: switching, buffering, releasing. J. Allergy Clin. Immunol. 113, 381–386.

    Article  PubMed  CAS  Google Scholar 

  • Vergara C. I., Acevedo N., Jiménez S., Martínez B., Mercado D., Gusmão L. et al. 2010 A Six-SNP Haplotype of ADAM33 Is Associated with Asthma in a Population of Cartagena, Colombia. Int. Arch. Allergy Immunol. 152, 32–40.

    Article  PubMed  CAS  Google Scholar 

  • Wang P., Liu Q. J., Li J. S., Li H. C., Wei C. H., Guo C. H. et al. 2006 Lack of association between ADAM33 gene and asthma in a Chinese population. Int. J. Immunogenet. 33, 303–306.

    Article  PubMed  CAS  Google Scholar 

  • Wenzel S. E. 2006 Asthma: defining of the persistent adult phenotypes. Lancet 368, 804–813.

    Article  PubMed  CAS  Google Scholar 

  • Werner M., Herbon N., Gohlke H., Altmüller J., Knapp M., Heinrich J. and Wjst M. 2004 Asthma is associated with single-nucleotide polymorphisms in ADAM33. Clin. Exp. Allergy 34, 26–31.

    Article  PubMed  CAS  Google Scholar 

  • Yoshinaka T., Nishii K., Yamada K., Sawada H., Nishiwaki E., Smith K. et al. 2002 Identification and characterization of novel mouse and human ADAM33s with potential metalloprotease activity. Gene 282, 227–236.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pediatrics, Chhtrapati Shahuji Maharaj Medical University, Chowk, Lucknow 226 003, India

    PRIYA TRIPATHI & SHALLY AWASTHI

  2. Department of Pulmonary Medicine, Chhtrapati Shahuji Maharaj Medical University, Chowk, Lucknow 226 003, India

    RAJENDRA PRASAD

  3. Department of Pathology, Chhtrapati Shahuji Maharaj Medical University, Chowk, Lucknow 226 003, India

    NUZHAT HUSAIN

  4. Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, 208 016, India

    SUBRAMANIAM GANESH

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  1. PRIYA TRIPATHI
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  2. SHALLY AWASTHI
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  3. RAJENDRA PRASAD
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  4. NUZHAT HUSAIN
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  5. SUBRAMANIAM GANESH
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Correspondence to SHALLY AWASTHI.

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Tripathi P., Awasthi S., Prasad R., Husain N. and Ganesh S. 2011 Association of ADAM33 gene polymorphisms with adult-onset asthma and its severity in an Indian adult population. J. Genet. 90, 265–273

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TRIPATHI, P., AWASTHI, S., PRASAD, R. et al. Association of ADAM33 gene polymorphisms with adult-onset asthma and its severity in an Indian adult population. J Genet 90, 265–273 (2011). https://doi.org/10.1007/s12041-011-0073-y

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  • DOI: https://doi.org/10.1007/s12041-011-0073-y

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