Abstract
Susceptibility to ‘scrapie’ disease in goats is influenced by polymorphisms of the prion protein (PRNP) gene. The aim of this study was to identify PRNP gene polymorphisms in a total of 356 scrapie disease-free goats from 10 Turkish native breeds. Eighteen single-nucleotide polymorphisms were detected in the caprine PRNP open-reading frame. Ten previously described amino acid substitutions (I142M, H143R, N146S, N146D, R151H, R154H, P168Q, R211Q, Q222K and P240S) and two novel dimorphisms (G134E and Q163P) were identified. The strongest association between caprine PRNP and relative resistance to scrapie disease has been reported previously for polymorphisms at codons 146 (S /D) and 222 (K). In the present study, these three PrP variants were relatively rare with 6.3%. This is the first report on PRNP gene variation in Turkish native goat breeds and our knowledge of these polymorphisms will assist goat breeding programmes to reduce the risk of scrapie.
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References
Acutis P. L., Bossers A., Priem J., Riina M. V., Peletto S., Mazza M. et al. 2006 Identification of prion protein gene polymorphisms in goats from Italian scrapie outbreaks. J. Gen. Virol. 87, 1029–1033.
Acutis P. L., Martucci F., D’Angelo A., Peletto S., Colussi S., Maurella C. et al. 2012 Resistance to classical scrapie in experimentally challenged goats carrying mutation K222 of the prion protein gene. Vet. Res. 43, 8.
Aguilar-Calvo P., Espinosa J. C., Pintado B., Gutiérrez-Adán A., Alamillo E., Miranda A. et al. 2014 Role of the goat K222- PrPC polymorphic variant in prion infection resistance. J. Virol. 88, 2670–2676.
Aguilar-Calvo P., Fast C., Tauscher K., Espinosa J. -C., Groschup M. H., Nadeem M. et al. 2015 Effect of Q211 and K222 PRNP polymorphic variants in the susceptibility of goats to oral infection with Goat-BSE. J. Infect. Dis. 212, 664–672.
Barillet F., Mariat D., Amigues Y., Faugeras R., Caillat H., Moazami-Goudarzi K. et al. 2009 Identification of seven haplotypes of the caprine PrP gene at codons 127, 142, 154, 211, 222 and 240 in French Alpine and Saanen breeds and their association with classical scrapie. J. Gen. Virol. 90, 769–776.
Benestad S. L., Arsac J. N., Goldmann W. and Nöremark M. 2008 Atypical /Nor98 scrapie: properties of the agent, genetics, and epidemiology. Vet. Res. 39, 19.
Billinis C., Panagiotidis C. H., Psychas V., Argyroudis S., Nicolaou A., Leontides S. et al. 2002 Prion protein gene polymorphisms in natural goat scrapie. J. Gen. Virol. 83, 713–721.
Bouzalas I. G., Dovas C. I., Banos G., Papanastasopoulou M., Kritas S., Oevermann A. et al. 2010 Caprine PRNP polymorphisms at codons 171, 211, 222 and 240 in a Greek herd and their association with classical scrapie. J. Gen. Virol. 91, 1629–1634.
Colussi S., Vaccari G., Rasero R., Maroni Ponti A., Ru G., Sacchi P. et al. 2010 Prospects for applying breeding for resistance to control scrapie in goats: the current situation in Italy. Small Rumin. Res. 88, 97–101.
Corbiere F., Perrin-Chauvineau C., Lacroux C., Costes P., Thomas M., Bremaud I. et al. 2013 PrP-associated resistance to scrapie in five highly infected goat herds. J. Gen. Virol. 94, 241–245.
Excoffier L., Laval G. and Schneider S. 2005 Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol. Bioinformatics 1, 47–50.
Goldmann W. 2008 PrP genetics in ruminant transmissible spongiform encephalopathies. Vet. Res. 39, 30.
Goldmann W., Martin T., Foster J., Hughes S., Smith G., Hughes K. et al. 1996 Novel polymorphisms in the caprine PrP gene: a codon 142 mutation associated with scrapie incubation period . J. Gen. Virol. 77, 2885–2891.
González L., Martin S., Sisó S., Konold T., Ortiz-Peláez A., Phelan L. et al. 2009 High prevalence of scrapie in a dairy goat herd: tissue distribution of disease-associated PrP and effect of PRNP genotype and age. Vet. Res. 40, 65.
Gürsoy O. 2006 Economics and profitability of sheep and goat production in Turkey under new support regimes and market conditions. Small Rumin. Res. 62, 181–191.
Hunter N. 1997 PrP genetics in sheep and the implications for scrapie and BSE. Trends Microbiol. 5, 8.
Huson D. H. and Bryant D. 2006 Application of phylogenetic networks in evolutionary studies. Mol. Biol. Evol. 23, 254–267.
Librado P. and Rozas J. 2009 DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinform. 25, 1451–1452.
Maestrale C., Cancedda M. G., Pintus D., Masia M., Nonno R., Ru G. et al. 2015 Genetic and pathological follow-up study of goats experimentally and naturally exposed to a sheep scrapie isolate. J. Virol. 89, 10044–10052.
Meydan H., Yüceer B., Degirmenci R., Özkan M. M. and Yildiz M. A. 2012 Prion protein gene polymorphism and genetic risk evaluation for scrapie in all Turkish native sheep breeds. Virus Genes. 45, 169–175.
Meydan H., Özkan M. M., Yildiz M. A. and Goldmann W. 2013a Novel polymorphism in ovine prion protein gene. Anim. Genet. 44, 588–591.
Meydan H., Özkan M. M. and Yildiz M. A. 2013b Genetic risk assessment for atypical scrapie in Turkish native sheep breeds. Small Rumin. Res. 111, 16–22.
Miller S. A., Dykes D. D. and Polesk H. F. 1988 A simple salting out procedure for extracting DNA from human nucleated cells. Nucl. Acids. Res. 16, 1215.
OIE 2010 World Organisation for Animal Health. Terrestrial Animal Health Code, Chapter 14.9. Scrapie (http://www.oie.int).
Ortiz-Pelaez A., Georgadou S., Simmons M. M., Windl O., Dawson M., Arnold M. E. et al. 2014 Allelic variants at codon 146 in the PRNP gene show significant differences in the risk for natural scrapie in Cypriot goats. Epidemiol. Infect. 143, 1304–1310.
Papasavva-Stylianou P., Windl O., Saunders G., Mavrikiou P., Toumazos P. and Kakoyiannis C. 2011 PrP gene polymorphisms in Cyprus goats and their association with resistance or susceptibility to natural scrapie. Vet. J. 187, 245–250.
Prusiner S. B. 2004 Prion biology and diseases, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA.
Vaccari G., Di Bari M. A., Morelli L., Nonno R., Chiappini B., Antonucci G. et al. 2006 Identification of anallelic variant of the goat PrP gene associated with resistance to scrapie. J. Gen. Virol. 87, 1395–1402.
Yilmaz O., Kor A., Ertugrul M. and Wilson R. T. 2012 The domestic livestock resources of Turkey-Goat breeds and types and their conservation Resources. Anim. Genet. Resources. 51, 105–116.
White S. N., Reynolds J. O., Waldron D. F., Schneider D. A. and O’Rourke K. I. 2012 Extended scrapie incubation time in goats singly heterozygous for PRNP S146 or K222. Gene 50, 49–51.
Zeder M. A. 2008 Domestication and early agriculture in the Mediterranean basin, origins, diffusion and impact. Proc. Natl. Acad. Sci. USA 105, 11597–11604.
Acknowledgments
The authors would like to thank Dr M. İriadam and Dr Birol Dağ for their assistance in the collection of samples. This study was conducted at Ankara University, Faculty of Agriculture, Animal Sciences, Ankara, Turkey, and was partially supported by a grant from Scientific Research Projects (BAP) of Ankara University to MMÖ (project no. BAP-12B4347008) and the Biotechnology and Biological Sciences Research Council, UK to WG (strategic programme grant no. BB /J004332 /1 to the Roslin Institute).
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Corresponding editor: Indrajit Nanda
[Meydan H., Pehlivan E., Özkan M. M., Yildiz M. A. and Goldmann W. 2017 Prion protein gene polymorphisms in Turkish native goat breeds. J. Genet. 96, xx–xx]
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MEYDAN, H., PEHLİVAN, E., ÖZKAN, M.M. et al. Prion protein gene polymorphisms in Turkish native goat breeds. J Genet 96, 299–305 (2017). https://doi.org/10.1007/s12041-017-0763-1
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DOI: https://doi.org/10.1007/s12041-017-0763-1