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Illumina-based de novo transcriptome sequencing and analysis of Chinese forest musk deer

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Abstract

The Chinese forest musk deer (Moschus berezovskii Flerov) is an endangered artiodactyl mammal. The musk secreted by sexually mature males is highly valued for alleged pharmaceutical properties and perfume manufacturing. However, the genomic and transcriptomic resources of musk deer remain deficiently represented and poorly understood. Next-generation sequencing technique is an efficient method for generating an enormous amount of sequence data that can represent a large number of genes and their expression levels. In the present study, we used Illumina HiSeq technology to perform de novo assembly of heart and musk gland transcriptomes from the Chinese forest musk deer. A total of 239,383 transcripts and 176,450 unigenes were obtained, of which 37,329 unigenes were matched to known sequences in the NCBI nonredundant protein (Nr) database; 31,039 unigenes were assigned to 61 GO terms, and 11,782 to 332 KEGG pathways. Additionally, 592 and 2282 differentially expressed genes were found to be specifically expressed in the heart and musk gland, respectively. The abundant transcriptomic data generated in the present report will provide a comprehensive sequence resource for Chinese forest musk deer as well as lay down a foundation which will help in accelerating genetic and functional genomics research in this species.

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Acknowledgements

This study was supported by National Natural Science Foundation of China (NSFC31672396 and NSFC81503188); the programme from Sichuan Agricultural University (02920400) and Sichuan Provincial Department of Science and Technology Programme (2015JQO023).

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Author notes

  1. Zhongxian Xu and Hang Jie contributed equally to this work.

Authors and Affiliations

  1. Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu,  611130, People’s Republic of China

    Zhongxian Xu, Binlong Chen, Uma Gaur, Nan Wu, Jian Gao, Mingyao Yang & Diyan Li

  2. Laboratory of Medicinal Animal, Chongqing Institute of Medicinal Plant Cultivation, Nanchuan, Chongqing, 408435, People’s Republic of China

    Hang Jie, Pinming Li, Guijun Zhao & Dejun Zeng

Authors
  1. Zhongxian Xu
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  2. Hang Jie
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  3. Binlong Chen
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  10. Mingyao Yang
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  11. Diyan Li
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Corresponding author

Correspondence to Diyan Li.

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Corresponding editor: Indrajit Nanda

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Xu, Z., Jie, H., Chen, B. et al. Illumina-based de novo transcriptome sequencing and analysis of Chinese forest musk deer. J Genet 96, 1033–1040 (2017). https://doi.org/10.1007/s12041-017-0872-x

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  • DOI: https://doi.org/10.1007/s12041-017-0872-x

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