Abstract
To evaluate whether EBV-DNA can be used as a diagnostic and follow-up parameter for nasopharyngeal tumors in a non-endemic population. The study was carried out in a university hospital. A retrospective study was conducted on 40 paraffin samples of histological preparations. EB-DNA was detected by real-time PCR technique. A prospective study was also conducted on a group of 30 patients who underwent nasopharyngeal biopsy for suspected nasopharyngeal carcinoma (NPC) by comparing EBV-DNA concentrations between the histological specimen and the serum. Quantification of genomic copies of EBV-DNA in serum and detection of anti-EBV antibodies was performed. In both groups the presence of high viral load of EBV-DNA was found in nonkeratinizing squamous cell carcinomas, in three cases of lymphepitomyoma and in 4 out of 6 cases of non-differentiated non-carcinoma lymph node metastases. squamous keratinizing cells. In all cases of NPC, an antibody pattern typical of reactivations (IgGVCA+, IgG-EA+, IgG-EBNA+) and IgA-EA-D, frequently positive in cases of NPC, has been highlighted. A good correlation between the high EBV-DNA charges and the histological diagnosis was highlighted. Our study also found that the assessment of viral EBV load can also be considered in the prognostic evaluation and in the follow-up of patients with NPC.
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Cabibi D, Speciale R, Lorusso F (2013) Recurrent ossifying fibroma of the maxillary sinus in an adult patient. Pathologica 105(1):11–14
Lo Casto A, Lorusso F, Lombardo F, Speciale R (2014) Secondary septal mucocele diagnosed by MRI and CBCT and treated surgically. B-ENT 10:221–225
Cheng WM, Chan KH, Chen HL, Luo RX, Ng SP, Luk W et al (2002) Assessing the risk of nasopharyngeal carcinoma on the basis of EBV antibody spectrum. Int J Cancer 97(4):489–492
Young LS, Rickinson AB (2004) Epstein-Barr virus: 40 years on. Nat Rev Cancer 4:757–768
Perez CA, Devineni VR, Marcial-Vega V, Marks JE, Simpson JR, Kucik N (1992) Carcinoma of the nasopharynx: factors affecting prognosis. Int J Radiat Oncol Biol Phys 23:271–280
Chan KC, Lo YM (2002) Circulating EBV DNA as a tumor marker for nasopharyngeal carcinoma. Semin Cancer Biol 12:489–496
Lung ML, Chang GC (1992) Detection of distinct Epstein-Barr virus genotypes in NPC biopsies from southern Chinese and Caucasians. Int J Cancer 52(1):34–37
Busson P, Keryer C, Ooka T, Corbex M (2004) EBV-associated nasopharyngeal carcinomas: from epidemiology to virus-targeting strategies. Trends Microbiol 12:356–360
Feng BJ, Huang W, Shugart YY et al (2002) Genome-wide scan for familial nasopharyngeal carcinoma reveals evidence of linkage to chromosome 4. Nat Genet 31:395–399
Fasciana T, Capra G, Calà C, Zambuto S, Mascarella C, Colomba C, Di Carlo P, Giammanco A (2017) Helicobacter pylori and Epstein-Barr co-infection in gastric disease. Pharmacol Online Open Access Spec Issue 1:73–82
Giardina A, Rizzo A, Ferrante A, Capra G, Triolo G, Ciccia F (2013) Giant cell arteritis associated with chronic active Epstein-Barr virus infection (Article) Reumatismo Open. Access 1:36–39
Di Carlo P, Trizzino M, Titone L, Capra G, Colletti P, Mazzola G, Pistoia D, Sarno C (2011) Unusual MRI findings in an immunocompetent patient with EBV encephalitis: a case report. BMC Med Imaging 11:6. https://doi.org/10.1186/1471-2342-11-6
Vokes EE, Liebowitz DN, Weichselbaum RR (1997) Nasopharyngeal carcinoma. Lancet 350:1087–1091
McDermott AL, Dutt SN, Watkinson JC (2001) The aetiology of nasopharyngeal carcinoma. Clin Otolaryngol 26:82–92
Thorley-Lawson DA (2001) Epstein-Barr virus: exploiting the immune system. Nat Rev Immunol 1:75–82
Hsu JL, Glaser SL (2000) Epstein-Barr virus-associated malignancies: epidemiologic patterns and etiologic implications. Crit Rev Oncol Hematol 34(1):27–53
de-Thé G, Day NE, Geser A et al (1975) Sero-epidemiology of the Epstein-Barr virus: preliminary analysis of an international study–a review. IARC Sci Publ. 11(2):3–16
Tierney RJ, Steven N, Young LS, Rickinson AB (1994) Epstein-Barr virus latency in blood mononuclear cells: analysis of viral gene transcription during primary infection and in the carrier state. J Virol 68:7374–7385
Thorley-Lawson DA, Gross A (2004) Persistence of the Epstein-Barr virus and the origins of associated lymphomas. N Engl J Med 350:1328–1337
Borza CM, Hutt-Fletcher LM (2002) Alternate replication in B cells and epithelial cells switches tropism of Epstein-Barr virus. Nat Med 8:594–599
Tugizov SM, Berline JW, Palefsky JM (2003) Epstein-Barr virus infection of polarized tongue and nasopharyngeal epithelial cells. Nat Med 9:307–314
Shannon-Lowe CD, Neuhierl B, Baldwin G, Rickinson AB, Delecluse HJ (2006) Resting B cells as a transfer vehicle for Epstein-Barr virus infection of epithelial cells. Proc Natl Acad Sci USA 103:7065–7070
Dowd JB, Palermo T, Brite J et al (2013) Seroprevalence of Epstein-Barr virus infection in U.S. children ages 6–19, 2003–2010. PLoS ONE 8(5):e64921
Murphy G, Pfeiffer R, Camargo MC et al (2009) Meta-analysis shows that prevalence of Epstein-Barr virus-positive gastric cancer differs based on sex and anatomic location. Gastroenterology 137(3):824–833
Stevens SJC, Verkuijlen SAWM, Hariwiyanto B, Harijadi, Fachiroh J, Paramita D, Tan IB, Haryana SM, Middeldorp JM (2005) Diagnostic value of measuring Epstein-Barr virus (EBV) DNA load and carcinoma-specific viral mRNA in relation to anti-EBV immunoglobulin A (IgA) and IgG antibody levels in blood of nasopharyngeal carcinoma patients from Indonesia. J Clin Microbiol 43:3066–3073
Chan KC, Zhang J, Chan AT, Lei KI, Leung SF, Chan LY, Chow KC, Lo YM (2003) Molecular characterization of circulating EBV DNA in the plasma of nasopharyngeal carcinoma and lymphoma patients. Cancer Res 63:2028–2032
Mutirangura A, Pornthanakanem W, Theamboonlers A, Sriuranpong V, Lertsanguansinchi P, Yenrudi S et al (1998) Epstein-Barr viral DNA in serum of patients with nasopharyngeal carcinoma. Clin Cancer Res 4:665–669
Chien YC, Chen JY, Liu MY, Yang HI, Hsu MM, Chen CJ, Yang CS (2001) Serologic markers of Epstein-Barr virus infection and nasopharyngeal carcinoma in Taiwanese men. N Engl J Med 345:1877–1882
Bejarano MT, Masucci MG (1998) Interleukin-10 abrogates the inhibition of Epstein-Barr virus-induced B-cell transformation by memory T-cell responses. Blood 92(11):4256–4262
Feng P, Chan SH, Soo MY, Liu D, Guan M, Ren EC, Hu H (2001) Antibody response to Epstein-Barr virus Rta protein in patients with nasopharyngeal carcinoma: a new serologic parameter for diagnosis. Cancer. 92(7):1872–1880
Lo YM, Leung SF, Chan LY, Lo KW, Zhang J, Chan AT et al (2000) Plasma cell-free Epstein-Barr virus DNA quantitation in patients with nasopharyngeal carcinoma. Correlation with clinical staging. Ann N Y Acad Sci 906:99–101
Chang KP, Hsu CL, Chang YL, Tsang NM, Chen CK, Lee TJ et al (2008) Complementary serum test of antibodies to Epstein-Barr virus nuclear antigen-1 and early antigen: a possible alternative for primary screening of nasopharyngeal carcinoma. Oral Oncol 44(8):784–792
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Lorusso, F., Caleca, M.P., Bellavia, C. et al. The EBV-DNA Can be Used as a Diagnostic and Follow-up Parameter of the Rhinopharyngeal Tumors in the Non-Endemic Population of the Western Sicily. Indian J Otolaryngol Head Neck Surg 71, 396–400 (2019). https://doi.org/10.1007/s12070-018-1427-z
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DOI: https://doi.org/10.1007/s12070-018-1427-z