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Mucoepidermoid Carcinoma Does Not Harbor Transcriptionally Active High Risk Human Papillomavirus Even in the Absence of the MAML2 Translocation

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Abstract

High risk human papillomavirus (HPV) is firmly established as an important cause of oropharyngeal carcinoma. Recent studies have also implicated HPV as a cause of mucoepidermoid carcinoma (MEC)—a tumor of salivary gland origin that frequently harbors MAML2 translocations. The purpose of this study was to determine the prevalence of transcriptionally active HPV in a large group of MECs and to determine whether HPV infection and the MAML2 translocation are mutually exclusive events. Break-apart fluorescence in situ hybridization for MAML2 was performed on a tissue microarray containing 92 MECs. HPV testing was performed using RNA in situ hybridization targeting high risk HPV mRNA E6/E7 transcripts. Of the 71 MECs that could be evaluated by FISH, 57 (80 %) harbored the MAML2 rearrangement. HPV was not detected in any of the 57 MECs that contained a MAML2 rearrangement, in any of the 14 MECs that did not contain the rearrangement, or in any of the 21 MECs where MAML2 status was unknown. High risk HPV does not appear to play any significant role in the development of MEC. It neither complements nor replaces MAML2 translocation in the tumorigenesis of MEC.

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Acknowledgments

This study was funded in part by the National Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR) Head and Neck SPORE Grant P50 DE019032.

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Authors and Affiliations

  1. Department of Pathology, The Johns Hopkins Medical Institutions, 401 N. Broadway, Weinberg 2242, Baltimore, MD, 21231, USA

    Justin A. Bishop, Raluca Yonescu, Denise Batista, Anna Yemelyanova & William H. Westra

  2. Department of Otolaryngology/Head and Neck Surgery, The Johns Hopkins Medical Institutions, Baltimore, MD, USA

    Justin A. Bishop, Patrick K. Ha & William H. Westra

  3. Department of Oncology, The Johns Hopkins Medical Institutions, Baltimore, MD, USA

    William H. Westra

  4. The Milton J. Dance, Jr. Head and Neck Center, The Greater Baltimore Medical Center, Baltimore, MD, USA

    Patrick K. Ha

Authors
  1. Justin A. Bishop
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  2. Raluca Yonescu
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  3. Denise Batista
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  4. Anna Yemelyanova
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  5. Patrick K. Ha
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  6. William H. Westra
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Correspondence to William H. Westra.

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Bishop, J.A., Yonescu, R., Batista, D. et al. Mucoepidermoid Carcinoma Does Not Harbor Transcriptionally Active High Risk Human Papillomavirus Even in the Absence of the MAML2 Translocation. Head and Neck Pathol 8, 298–302 (2014). https://doi.org/10.1007/s12105-014-0541-9

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  • DOI: https://doi.org/10.1007/s12105-014-0541-9

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