Abstract
This study describes a method development for the determination of rosmarinic acid (RA) by using a gradient high-performance liquid chromatography (HPLC) and its application to certain plant materials. The analysis was performed by utilizing a two solvents system [A: methanol/water/formic acid (10:88:2; v:v:v); B: methanol/water/formic acid (90:8:2; v:v:v)] on a reverse-phase column. The flow rate and injection volume were 1 ml min−1 and 10 μl, respectively. Signals were detected at 280 nm. In addition, an internal standard (IS) technique was applied for the analysis of RA to increase precision, and propylparaben was employed for this purpose. The repeatability results as RSD% were 1.66, 1.17 and 1.26 for intra-day and 1.38 was for inter-day with the employment of (3.67 × 10−5 M) RA. A limit of linearity (LOL) was observed in a wide (1.13 × 10−5–5.65 × 10−4 M) concentration range. Linearity parameters were also examined in the range of 5.95 × 10−6–7.14 × 10−5 M RA, and very good correlation was observed. The limit of detection (LOD) and limit of quantification (LOQ) (for inter-day) were 1.60 × 10−6 M (signal/noise [S/N] = 3.3) and 4.80 × 10−6 M (S/N = 10), respectively. The method was applied to the extracts of certain Lamiaceae plants (Salvia candidissima Vahl. subsp. candidissima, S. sclarea L., S. verticillata L. subsp. verticillata and R. officinalis L.), and reasonable results were obtained.
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Akowuah GA, Zhari I, Norhayati I, Sadikun A, Khamsah SM (2004) Food Chem 87:559
Andrade P, Ferreres F, Gil MI, Tomás-Barberán FA (1997) Food Chem 60:79
Bandoniene D, Murkovic M, Venskutonis PR (2005) J Chromatogr Sci 43:372
Baskan S, Öztekin N, Erim FB (2007) Food Chem 101:1748
Cuvelier ME, Richard H, Berset C (1996) J Am Oil Chem Soc 73:645
Exarchou V, Nenadis N, Tsimidou M, Gerothanassis IP, Troganis A, Boskou D (2002) J Agric Food Chem 50:5294
Ito H, Miyazaki T, Ono M, Sakurai H (1998) Bioorganic Med Chem 6:1051
Li X, Yu C, Cai Y, Liu G, Jia J, Wang Y (2005) J Chromatogr B 820:41
Liu AH, Li L, Xu M, Lin YH, Guo HZ, Guo DA (2006) J Pharm Biomed Anal 41:48
Lopez-Arnaldos T, Lopez-Serrano M, Ros Barcelo A, Calderon AA, Zapata JM (1995) J Anal Chem 351:311
Lu Y, Foo LY (1999) Phytochemistry 51:91
Masaki H, Sakaki S, Atsumi T, Sakurai H (1995) Biol Pharm Bull 18:162
Öztürk N, Tunçel M, Tunçel NB (2007) J Liq Chromatogr Relat Technol 30:587
Parejo I, Viladomat F, Bastida J, Codina C (2004) Anal Chim Acta 512:271
Parnham MJ, Kesselring K (1985) Drugs Future 10:756
Petersen M, Simmonds MSJ (2003) Phytochemistry 62:121
Pizzale L, Bortolomeazzi R, Vichi S, Überegger E, Conte LS (2002) J Sci Food Agric 82:1645
Santos-Gomes PC, Seabra RM, Andrade PB, Fernandes-Ferreira M (2002) J Plant Physiol 162:981
Scarpati ML, Oriente G (1958) Ric Sci 28:2329
Stehfest K, Boese M, Kerns G, Piry A, Wilhelm C (2004) J Plant Physiol 161:151
Sterbova D, Matejicek D, Vlcek J, Kuban V (2004) Anal Chim Acta 513:435
Tepe B (2008) Bioresour Technol 99:1584
Tepe B, Eminagaoglu Ö, Akpulat HA, Aydin E (2007) Food Chem 100:985
The International Conference on Harmonization (ICH) (1996) Topic Q 2 B Validation of Analytical Procedures: Methodology, CPMP/ICH/281/95
Troncoso N, Sierra H, Carvajal L, Delpiano P, Günther G (2005) J Chromatogr A 1100:20
Tsao R, Den Z (2004) J Chromatogr B 812:85
Wang H, Provan GJ, Helliwell K (2004) Food Chem 87:307
Ziakova A, Brandsteterova E, Blahova E (2003) J Chromatogr A 983:271
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The authors acknowledge the Anadolu University Scientific Research Projects (Project No: 030353) and the Plant, Drug and Scientific Research Centre of Anadolu University (AUBIBAM) for their kind support of this study.
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Öztürk, N., Tunçel, M., Uysal, U.D. et al. Determination of Rosmarinic Acid by High-Performance Liquid Chromatography and Its Application to Certain Salvia Species and Rosemary. Food Anal. Methods 4, 300–306 (2011). https://doi.org/10.1007/s12161-010-9164-2
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DOI: https://doi.org/10.1007/s12161-010-9164-2