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Selection and Application of DNA Aptamers Against Sulfaquinoxaline Assisted by Graphene Oxide–Based SELEX

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Abstract

Single-stranded DNA aptamers that specifically bind to sulfaquinoxaline (SQX) were selected by a graphene oxide–based SEL EX (GO-SELEX) technique assisted by a non-immobilizing TAMRA-labeled oligonucleotide library. After 8 rounds of selection against SQX, two aptamer candidates (i.e., SBA 1 and SBA 2) were obtained. Furthermore, the two full-length aptamers were truncated to obtain the aptamers (i.e. SBA 1-1, SBA 2-1, and SBA 2-2). These candidate aptamers were subjected to binding assays to evaluate their binding affinities and specificities to SQX. Our results show that the dissociation constants Kd of the aptamers ranged from 82.54 to 630.41 nM. Using truncated aptamer SBA 2-1 with the highest affinity as the recognition element, a GO-based fluorescent aptasensor was developed for SQX detection with a linear range from 0.05 to 50 ng mL−1 and a limit of detection of 0.11 ng mL−1 with excellent selectivity. Furthermore, the new aptasensor was used to detect SQX in milk samples. Our results showed that the aptasensor demonstrated recoveries ranging from 96.6 to 106.7%, suggesting that the proposed GO-based fluorescent aptasensor holds great potential as promising tool for sensitive detection of SQX in food safety inspection.

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Funding

The work was financially supported by the National Natural Science Foundation of China (Grant No. 31671939), the Science and Technology Research Program of Chongqing Municipal Education Commission (KJZD-K201800501), the Scientific and Technological Research Project of Chongqing (cstc2018jscx-msybX0201), and the Postgraduate Scientific Research and Innovation Project of Chongqing Municipal Education Commission (CYS19297).

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  1. College of Life Sciences, Chongqing Normal University, 37 Chengzhong Road, University Town, Chongqing, 401331, People’s Republic of China

    Haixing Shi, Qiming Kou, Ping Wu, Qi Sun, Juan Wu & Tao Le

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  1. Haixing Shi
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  2. Qiming Kou
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  3. Ping Wu
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  4. Qi Sun
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  5. Juan Wu
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  6. Tao Le
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Correspondence to Tao Le.

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Haixing Shi declares that he has no conflict of interest. Qiming Kou declares that he has no conflict of interest. Ping Wu declares that he has no conflict of interest. Qi Sun declares that he has no conflict of interest. Juan wu declares that he has no conflict of interest. Tao Le declares that he has no conflict of interest.

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Shi, H., Kou, Q., Wu, P. et al. Selection and Application of DNA Aptamers Against Sulfaquinoxaline Assisted by Graphene Oxide–Based SELEX. Food Anal. Methods 14, 250–259 (2021). https://doi.org/10.1007/s12161-020-01869-2

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