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Separating Fluid Shear Stress from Acceleration during Vibrations In Vitro: Identification of Mechanical Signals Modulating the Cellular Response

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Abstract

The identification of the physical mechanism(s) by which cells can sense vibrations requires the determination of the cellular mechanical environment. Here, we quantified vibration-induced fluid shear stresses in vitro and tested whether this system allows for the separation of two mechanical parameters previously proposed to drive the cellular response to vibration—fluid shear and peak accelerations. When peak accelerations of the oscillatory horizontal motions were set at 1 g and 60 Hz, peak fluid shear stresses acting on the cell layer reached 0.5 Pa. A 3.5-fold increase in fluid viscosity increased peak fluid shear stresses 2.6-fold while doubling fluid volume in the well caused a 2-fold decrease in fluid shear. Fluid shear was positively related to peak acceleration magnitude and inversely related to vibration frequency. These data demonstrated that peak shear stress can be effectively separated from peak acceleration by controlling specific levels of vibration frequency, acceleration, and/or fluid viscosity. As an example for exploiting these relations, we tested the relevance of shear stress in promoting COX-2 expression in osteoblast like cells. Across different vibration frequencies and fluid viscosities, neither the level of generated fluid shear nor the frequency of the signal were able to consistently account for differences in the relative increase in COX-2 expression between groups, emphasizing that other variables including out-of-phase accelerations of the nucleus may play a role in the cellular response to vibrations.

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Acknowledgments

Funding by the National Institutes of Health (NIAMS) is gratefully acknowledged. Technical expertise from Dr. Michael Hadjiargyrou and Lester Orlick was greatly appreciated.

Disclosures

Clinton Rubin is a founder of Marodyne Medical, Inc. Both Stefan Judex and Clinton Rubin own (provisional) patents regarding the application of vibrations to the musculoskeletal system.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, 11794, USA

    Gunes Uzer, Sarah L. Manske, M. Ete Chan, Clinton T. Rubin, Mary D. Frame & Stefan Judex

  2. Department of Mechanical Engineering, Stony Brook University, Stony Brook, NY, 11794, USA

    Fu-Pen Chiang

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  1. Gunes Uzer
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  2. Sarah L. Manske
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Correspondence to Stefan Judex.

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Associate Editor Edward Guo oversaw the review of this article.

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Uzer, G., Manske, S.L., Chan, M.E. et al. Separating Fluid Shear Stress from Acceleration during Vibrations In Vitro: Identification of Mechanical Signals Modulating the Cellular Response. Cel. Mol. Bioeng. 5, 266–276 (2012). https://doi.org/10.1007/s12195-012-0231-1

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