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Heavy metals biomonitoring via inhibitive assay of acetylcholinesterase from Periophthalmodon schlosseri

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Abstract

Acetylcholinester ase (AChE) generally known to be sensitive toward insecticides but its sensitivity toward heavy metals was least reported. Herein, a sensitive assay for heavy metals has been pursued using AChE in a rapid and economic manner. The AChE from a mudskipper, Periophthalmodon schlosseri has been found to be sensitive toward copper > mercury > chromium > and arsenic ions at the sub parts per million levels. Field trial works showed that the assay was applicable in detecting heavy metals pollution from effluents of industrial sites at near real time and verified using ICP-OES and Flow Injection Mercury System (FIMS 400). Furthermore, hierarchical cluster analyses of inhibition profiles were performed, revealing a comparable capability of the AChE compared to the gold standard of Microtox™ method.

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Acknowledgments

We thank the support of fund from Putra Research Grant under Project Number GP-IPM/2013/9401900.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia

    Mohd Khalizan Sabullah, Mohd Yunus Shukor & Amir Syahir

  2. School of Food Science and Nutrition, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia

    Mohd Khalizan Sabullah & Mohd Rosni Sulaiman

  3. Snoc International Sdn.Bhd, 1-2B Jalan Hentian 1B, Hentian Kajang, Jalan Reko, 43600, Kajang, Selangor, Malaysia

    Mohd Shukri Shukor

  4. Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia

    Mohd Termizi Yusof

  5. Department of Environmental Sciences, University Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia

    Wan Lutfi Wan Johari

Authors
  1. Mohd Khalizan Sabullah
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  2. Mohd Rosni Sulaiman
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  3. Mohd Shukri Shukor
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  4. Mohd Termizi Yusof
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  5. Wan Lutfi Wan Johari
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  6. Mohd Yunus Shukor
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  7. Amir Syahir
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Corresponding author

Correspondence to Amir Syahir.

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Sabullah, M.K., Sulaiman, M.R., Shukor, M.S. et al. Heavy metals biomonitoring via inhibitive assay of acetylcholinesterase from Periophthalmodon schlosseri . Rend. Fis. Acc. Lincei 26, 151–158 (2015). https://doi.org/10.1007/s12210-014-0359-0

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  • DOI: https://doi.org/10.1007/s12210-014-0359-0

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