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Bci528I, a new isoschizomer of EcoRI isolated from Bacillus circulans 528

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Abstract

Bacillus circulans 528 produces a restriction endonuclease, Bci528I which is an isoschizomer of EcoRI. We purified the enzyme, using Sephadex G-150, Phospho-cellulose, DEAE-cellulose, Hepharin-Sepharose CL-6B chromatography. The specific activity of Bci528I was 29,400 U/mg·protein. Bci528I recognizes 5′-GAATTC-3′ in dsDNA and cleaves between G and A of the recognition sequence, producing a symmetric four base 5′overhang.

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Acknowledgements

We thank Dr. Chol-Min Pak of the research institute of biotechnology, academy of science, the DPR of Korea for determination of recognition sequence and cleavage site.

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Authors and Affiliations

  1. Research Institute of Biotechnology, Faculty of Life Science, Kim Il Sung University, Pyongyang, Democratic People’s Republic of Korea

    Sung-Ryong Ra, Myong-Suk Kim, Chon-IL Paek, Yong-Chol Pak, Song-Hui Pak & Hyong-Bom Pak

  2. Korean Center for Culture Collection, Pyongyang, Democratic People’s Republic of Korea

    Kum-Chol Ri

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  1. Sung-Ryong Ra
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  2. Myong-Suk Kim
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  3. Chon-IL Paek
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  4. Yong-Chol Pak
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  5. Song-Hui Pak
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  6. Hyong-Bom Pak
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  7. Kum-Chol Ri
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Contributions

Sung-Ryong Ra conceived and designed the experiments. Myong-Suk Kim and Chon-Il Paek conducted experiments with support from Hyong-Bom Pak, Kum-Chol Ri, and Yong-Chol Pak. Song Hui Pak analyzed the data. Chon-Il Paek wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Sung-Ryong Ra.

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Ra, SR., Kim, MS., Paek, CI. et al. Bci528I, a new isoschizomer of EcoRI isolated from Bacillus circulans 528. Folia Microbiol 64, 803–808 (2019). https://doi.org/10.1007/s12223-019-00694-3

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  • DOI: https://doi.org/10.1007/s12223-019-00694-3

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