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Optimization of extraction and purification of glucoamylase produced by Aspergillus awamori in solid-state fermentation

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Abstract

Various parameters such as solvent selection, concentration, soaking time, and temperature were tested in a single bioreactor in order to determine optimum extraction conditions of glucoamylase, when produced simultaneously with protease by Aspergillus awamari nakazawa MTCC 6652. Optimum conditions were achieved in a 10% glycerol solution soaked for 2 h at 40°C, followed by concentration of extracted glucoamylase (9,157 U/gds) by acetone precipitation (1:2, v/v), which yielded 51.9% recovery. Ion exchange chromatography and gel filtration showed specific activities of 270.5 and 337.5 U/mg, respectively, while SDS-PAGE and zymogram analysis of glucoamylase indicated the presence of three starch-hydrolyzing isoforms with molecular weights of approximately 109.6, 87.1, and 59.4 kDa, respectively

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Authors and Affiliations

  1. Biotechnology Section, Applied Mechanics Department, Motilal Nehru National Institute of Technology, Allahabad, 211004, Uttar Pradesh, India

    Sangeeta Negi

  2. Agriculture and food Engineering Department, Microbial Biotechnology and Downstream Processing Laboratory, Indian Institute of Technology, Kharagpur, 721302, West Bengal, India

    Rintu Banerjee

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  1. Sangeeta Negi
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  2. Rintu Banerjee
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Correspondence to Sangeeta Negi.

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Negi, S., Banerjee, R. Optimization of extraction and purification of glucoamylase produced by Aspergillus awamori in solid-state fermentation. Biotechnol Bioproc E 14, 60–66 (2009). https://doi.org/10.1007/s12257-008-0107-3

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  • DOI: https://doi.org/10.1007/s12257-008-0107-3

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