Abstract
In this study, castor oil is hydrolyzed in presence of Candida rugosa lipase, while in the buffer (aqueous) phase as a dispersion medium. The following conditions were used to optimize the process: speed of agitation, initial pH of buffer phase, temperature, and ratio of buffer phase volume to oil weight. The optimal conditions are 1,100 rpm, pH 6.5, temperature 35°C, and 3:1 buffer phase volume to oil weight ratio. Under these described conditions, the reusability of lipase was tested and it was found that nearly 80% of original hydrolysis percentage was achieved after the first recycle.
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Goswami, D., Basu, J.K. & De, S. Optimization of process variables in castor oil hydrolysis by Candida rugosa lipase with buffer as dispersion medium. Biotechnol Bioproc E 14, 220–224 (2009). https://doi.org/10.1007/s12257-008-0123-3
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DOI: https://doi.org/10.1007/s12257-008-0123-3