Abstract
The optimization and kinetic modeling of curdlan synthesis by Agrobacterium radiobacter mutant A-15 under multi-physiological-parameters process control, especially employing the on-line oxygen uptake rate (OUR) and carbon dioxide evolution rate (CER) were performed in a 50-L fermentor. Results indicated that the maximum production of curdlan reach about 32.5 g/L with a glucose conversion yield of 49.5%, the maximum specific growth rate was about 0.5338 h−1, the kinetics equation were of positive significance for production. It also demonstrated that A-15 was insensitive to catabolite repression during polysaccharide production for its maintaining high conversion yield in the medium contained 6.6% (W/V) initial glucose. In addition, the OUR and CER at the stationary growth stage showed a positive correlation with curdlan synthesis. This work will provide more theoretical support for large-scale curdlan production.
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Acknowledgements
This work was supported by research grants from the Natural Science Foundation of Guangxi Province (CN) (2017GXNSFAA198128) and Shan Dong Fu yang biological technology Co., Ltd, China. The work was also thanks to the Shanghai (P.R. China) Guoqiang biochemical engineering equipment Co., Ltd, for providing the systems of automatic control fermentor supported with Biostar software.
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Yang, H., Li, W., Chen, S. et al. Optimization and Modeling of Curdlan Production under Multi-physiological-parameters Process Control by Agrobacterium radiobacter Mutant A-15 at High Initial Glucose. Biotechnol Bioproc E 26, 1012–1022 (2021). https://doi.org/10.1007/s12257-021-0028-y
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DOI: https://doi.org/10.1007/s12257-021-0028-y