Abstract
A bacterial strain 5YN5-8T was isolated from peat layer on Yongneup in Korea. Cells of strain 5YN5-8T were strictly aerobic, Gram-negative, coccobacilli, non-spore forming, and non-motile. The isolate exhibited optimal growth at 28°C, pH 7.0, and 0–1% NaCl. Results of 16S rRNA gene sequence analyses indicated a close relationship of this isolate to Acinetobacter calcoaceticus (97.8% similarity for strain DSM 30006T). It also exhibited 94.4–97.8% 16S rRNA gene sequence similarities to the validly published Acinetobacter species. The value for DNA-DNA hybridization between strain 5YN5-8T and other members of the genus Acinetobacter ranged from 16 to 28%. Predominant cellular fatty acids were C18:1 ω9c, summed feature 4 containing C15:0 iso 2-OH and/or C16:1 ω7c, and C16:0. The DNA G+C content was 43.9 mol%. Phylogenetic, phenotypic, and chemotaxonomic data accumulated in this study revealed that the isolate could be classified in a novel species of the genus Acinetobacter. The name Acinetobacter brisouii sp. nov. is proposed for the novel species, with 5YN5-8T (=KACC 11602T = DSM 18516T) as the type strain.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Breznak, J.A. and R.N. Costilow. 1994. Physicochemical factors in growth, pp. 137–154. In P. Gerhardt, R.G.E. Murray, W.A. Wood, and N.R. Krieg (eds.), Methods for General and Molecular Bacteriology-1994. American Society for Microbiology, Washington, D.C., USA.
Brisou, J. and A.R. Prévot. 1954. Études de systématique bactérienne. X. Révision des espèces réunies dans le genre Achromobacter. Annales de l’Institut Pasteur (Paris) 86, 722–728.
Carr, E.L., P. Kämpfer, B.K.C. Patel, V. Gürtler, and R.J. Seviour. 2003. Seven novel species of Acinetobacter isolated from activated sludge. Int. J. Syst. Evol. Microbiol. 53, 953–963.
Felsenstein, J. 1981. Evolutionary trees from DNA sequences: a maximum likelihood approach. J. Mol. Evol. 17, 368–376.
Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.
Fitch, W.M. 1971. Toward defining the course of evolution: minimum change for a specific tree topology. Syst. Zool. 20, 406–416.
Juni, E. 1984. Genus III. Acinetobacter Brisou et Prévot 1954, pp. 303–307. In N.R. Krieg and J.G. Holt (eds.), Bergey’s Manual of Systematic Bacteriology, vol. 1. The Williams & Wilkins Co., Baltimore, Maryland, USA.
Kim, D., K.S. Baik, M.S. Kim, S.C. Park, S.S. Kim, M.S. Rhee, Y.S. Kwak, and C.N. Seong. 2008. Acinetobacter soli sp. nov., isolated from forest soil. J. Microbiol. 46, 396–401.
Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge: Cambridge University Press, UK.
Kumar, S., K. Tamura, and M. Nei. 2004. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief. Bioinform. 5, 150–163.
Kwon, S.W., J.S. Kim, I.C. Park, S.H. Yoon, D.H. Park, C.K. Lim, and S.J. Go. 2003. Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int. J. Syst. Evol. Microbiol. 53, 21–27.
Lee, J.S., K.C. Lee, K.K. Kim, I.C. Hwang, C. Jang, N.G. Kim, W.H. Yeo, B.S. Kim, Y.M. Yu, and J.S. Ahn. 2009. Acinetobacter antiviralis sp. nov., from tobacco plant roots. J. Microbiol. Biotechnol. 19, 250–256.
Mesbah, M., U. Premachandran, and W.B. Whitman. 1989. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol. 39, 159–167.
Nishimura, Y., T. Ino, and H. Iizuka. 1988. Acinetobacter radioresistens sp. nov. isolated from cotton and soil. Int. J. Syst. Bacteriol. 38, 209–211.
Reasoner, D.J. and E.E. Geldreich. 1985. A new medium for the enumeration and subculture of bacteria from potable water. Appl. Environ. Microbiol. 49, 1–7.
Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.
Seldin, L. and D. Dubnau. 1985. Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int. J. Syst. Bacteriol. 35, 151–154.
Smibert, R.M. and N.R. Krieg. 1994. Phenotypic characterization, pp. 607–654. In P. Gerhardt, R.G.E. Murray, W.A. Wood, and N.R. Krieg (eds.), Methods for General and Molecular Bacteriology. American Society for Microbiology, Washington, D.C., USA.
Thompson, J.D., D.G. Higgins, and T.J. Gibson. 1994. Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673–4680.
Wayne, L.G., D.J. Brenner, R.R. Colwell, P.A.D. Grimont, O. Kandler, M.J. Krichevsky, L.H. Moore, W.E.C. Moore, R.G.E. Murray, E. Stackebrandt, M.P. Starr, and H.G. Truper. 1987. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37, 463–464.
Yoon, J.H., I.G. Kim, and T.K. Oh. 2007. Acinetobacter marinus sp. nov. and Acinetobacter seohaensis sp. nov., isolated from sea water of the yellow sea in Korea. J. Microbiol. Biotechnol. 17, 1743–1750.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Anandham, R., Weon, HY., Kim, SJ. et al. Acinetobacter brisouii sp. nov., isolated from a wetland in Korea. J Microbiol. 48, 36–39 (2010). https://doi.org/10.1007/s12275-009-0132-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12275-009-0132-8