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Acinetobacter baumannii outer membrane protein a modulates the biogenesis of outer membrane vesicles

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Abstract

Acinetobacter baumannii secretes outer membrane vesicles (OMVs) during both in vitro and in vivo growth, but the biogenesis mechanism by which A. baumannii produces OMVs remains undefined. Outer membrane protein A of A. baumannii (AbOmpA) is a major protein in the outer membrane and the C-terminus of AbOmpA interacts with diaminopimelate of peptidoglycan. This study investigated the role of AbOmpA in the biogenesis of A. baumannii OMVs. Quantitative and qualitative approaches were used to analyze OMV biogenesis in A. baumannii ATCC 19606T and an isogenic ΔAbOmpA mutant. OMV production was significantly increased in the ΔAbOmpA mutant compared to wild-type bacteria as demonstrated by quantitation of proteins and lipopolysaccharides (LPS) packaged in OMVs. LPS profiles prepared from OMVs from wild-type bacteria and the ΔAbOmpA mutant had identical patterns, but proteomic analysis showed different protein constituents in OMVs from wild-type bacteria compared to the ΔAbOmpA mutant. In conclusion, AbOmpA influences OMV biogenesis by controlling OMV production and protein composition.

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Author notes

  1. Chul Hee Choi

    Present address: Department of Periodontology, University of Florida, Gainesville, Florida, USA

Authors and Affiliations

  1. Department of Microbiology, Kyungpook National University School of Medicine, Daegu, 700-422, Republic of Korea

    Dong Chan Moon, Chul Hee Choi, Jung Hwa Lee & Je Chul Lee

  2. Division of Life Science, Korea Basic Science Institute, Daejeon, 305-806, Republic of Korea

    Chi-Won Choi & Seung Il Kim

  3. Division of Magnetic Resonance Research, Korea Basic Science Institute, Chungbuk, 363-883, Republic of Korea

    Hye-Yeon Kim & Jeong Soon Park

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  1. Dong Chan Moon
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  2. Chul Hee Choi
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Correspondence to Seung Il Kim or Je Chul Lee.

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These authors contributed equally to this work.

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Moon, D.C., Choi, C.H., Lee, J.H. et al. Acinetobacter baumannii outer membrane protein a modulates the biogenesis of outer membrane vesicles. J Microbiol. 50, 155–160 (2012). https://doi.org/10.1007/s12275-012-1589-4

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