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Effect of Asymptomatic Plasmodium falciparum Parasitaemia on Platelets Thrombogenicity in Blood Donors

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Indian Journal of Hematology and Blood Transfusion Aims and scope Submit manuscript

Abstract

Currently, blood donors in Ghana are not screened for malaria parasites. Therefore, this study assessed platelet thrombogenicity in blood donors infected asymptomatically with Plasmodium falciparum and the relationship between tumour necrosis factor alpha (TNF-α), 8-iso-prostaglandin F2α oxidative stress biomarker (8-iso-PG2α), C-reactive protein (hs-CRP) and D-dimer, and platelet thrombogenes levels. Haematology analyser was used to enumerate platelet count and platelet indices in 80 P. falciparum infected blood donors and 160 matched non-infected controls. Replicate serum levels of von Willebrand Factor (vWF), platelet factor 4 (PF4), P-selectin thrombogenic factors as well as TNF-α and 8-iso-PG2α were determined using enzyme immuno-assay while high sensitive hs-CRP and D-dimer concentrations were determined by fluorescent immunoassay. The geometric mean of parasite density in malaria infected donors was 1784 parasites/µL (505-2478 parasites/µL). This led to significant increase in the mean levels of 8-iso-PG2α, hs-CRP, TNF-α and D-dimer. However, PF4, P-selectin were significantly lower in infected donors while vWF levels did not differ significantly among the groups even though lower levels were observed in the infected donors. Significant direct relationship existed between both P-selectin and PF4 and platelet count, and plateletcrit and platelet large cell ratio whereas these thrombogenic factors varied inversely to 8-iso-PG2α, TNF-α and hs-CRP. Relative thrombocytopaenia was associated with significant reduction in P-selectin and platelet factor 4 levels together with increased 8-iso-PG2α, hs-CRP, TNF-α and D-dimer levels. Taken together, it is recommended that all P. falciparum infected blood donors should be deferred.

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Data Availability

The data used to support the findings of this study have been deposited in the Harvard Dataverse repository (https://doi.org/10.7910/DVN/UGYOQF).

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Acknowledgements

The authors are grateful to staff of National Blood Service, Ghana for collaborating with us to implement this study. We are also grateful to National Malaria Control Programme, Ghana for donating the malaria diagnostic kits used for the study.

Funding

This work was funded from authors’ own resources.

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Authors and Affiliations

  1. School of Basic and Biomedical Sciences, Department of Biomedical Sciences, University of Health and Allied Sciences, PMB 31, Ho, Ghana

    Enoch Aninagyei

  2. Department of Medical Laboratory Science, School of Allied Health Sciences, University of Cape Coast, Cape Coast, Ghana

    Patrick Adu

  3. Ghana Field Epidemiology and Laboratory Programme, School of Public Health, University of Ghana, Legon, Accra, Ghana

    Tanko Rufai

  4. Department of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Cape Coast, Ghana

    Paulina Ampomah, Godwin Kwakye-Nuako, Alexander Egyir-Yawson & Desmond Omane Acheampong

Authors
  1. Enoch Aninagyei
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  2. Patrick Adu
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  4. Paulina Ampomah
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  5. Godwin Kwakye-Nuako
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  6. Alexander Egyir-Yawson
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  7. Desmond Omane Acheampong
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Contributions

EA, AE-Y, DOA conceptualized, supervised the study and analysed study data. PA, TR, PA, GK-N collected donor specimens and performed laboratory analysis. Manuscript was drafted by EA, DOA, GK-Nand was reviewed and approved by all authors.

Corresponding authors

Correspondence to Enoch Aninagyei or Desmond Omane Acheampong.

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The authors declare that they have no conflict of interest.

Ethics Approval

This study was approved by Ghana Health Service Ethical Review Committee (GHS-REC002/03/18). The procedures used in this study adhere to the tenets of the Declaration of Helsinki.

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Aninagyei, E., Adu, P., Rufai, T. et al. Effect of Asymptomatic Plasmodium falciparum Parasitaemia on Platelets Thrombogenicity in Blood Donors. Indian J Hematol Blood Transfus 37, 632–639 (2021). https://doi.org/10.1007/s12288-020-01390-w

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  • DOI: https://doi.org/10.1007/s12288-020-01390-w

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