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Correlation of Clinical and Histopathological Grades in Oral Submucous Fibrosis Patients with Oxidative Stress Markers in Saliva

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Abstract

This study aimed to correlate the oxidative stress marker levels in saliva with the clinical stage based on mouth opening, fibrotic bands and histopathological grades of oral submucous fibrosis (OSF) patients. The study included patients clinically diagnosed with OSF (n = 63) and equal number of age and gender matched controls. Patients with OSF were defined by mouth opening stage, fibrotic bands and histopathological grades. Unstimulated saliva from both control and OSF patients were analysed for oxidative markers like lipid peroxides (LPO), non-enzymic antioxidants [reduced glutathione (GSH), vitamin A, vitamin E, vitamin C] and enzymatic antioxidants [glutathione peroxidase (GPx), superoxide dismutase (SOD)] and correlated with different stages and grades. Total salivary protein and LPO were significantly increased in OSF group with no significant change in the levels of GSH compared to controls. In OSF patients, a significant decrease in the levels of vitamins A, C and E was observed. The activities of salivary SOD and GPx were significantly decreased in OSF patients compared to controls. These changes significantly correlated with the increasing and differing grades of OSF that reflects increased oxidative stress with the progress of OSF.

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Acknowledgements

The study was funded by Young Faculty Research Grant of Sri Ramachandra University (2010–2011).

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Authors and Affiliations

  1. Department of Oral Medicine and Radiology, Faculty of Dental Sciences, Sri Ramachandra University, Porur, Chennai, 600 116, India

    C. V. Divyambika & S. Sathasivasubramanian

  2. Department of Biochemistry, University of Madras, Guindy Campus, Chennai, 600 025, India

    G. Vani & A. J. Vanishree

  3. Department of Oral Pathology, Faculty of Dental Sciences, Sri Ramachandra University, Porur, Chennai, 600 116, India

    N. Malathi

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  1. C. V. Divyambika
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  2. S. Sathasivasubramanian
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  3. G. Vani
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  5. N. Malathi
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Correspondence to G. Vani.

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Divyambika, C.V., Sathasivasubramanian, S., Vani, G. et al. Correlation of Clinical and Histopathological Grades in Oral Submucous Fibrosis Patients with Oxidative Stress Markers in Saliva. Ind J Clin Biochem 33, 348–355 (2018). https://doi.org/10.1007/s12291-017-0689-7

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  • DOI: https://doi.org/10.1007/s12291-017-0689-7

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