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Characterization of phytochemicals and evaluation of anti-cancer potential of Blumea eriantha DC.

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Abstract

The aim of the present study was to evaluate the in vitro anti-cancer and anti-oxidant potential of methanol extract of Blumea eriantha as well as its phytochemical characterization. The in vitro cytotoxic and antiproliferative activities of B. eriantha methanolic extract of leaves were evaluated using MTT assay on HeLa and B16F10 cell lines, and wound scratch and colony formation assays on B16F10 cell lines. The expressions of p53 and Bcl-2 genes were also determined by reverse transcriptase-polymerase chain reaction to establish apoptosis. Isolation and identification of chemical constituents were carried out by various chromatographic and spectroscopic analytical techniques including HPTLC and LC-MS. The methanol extract of the leaves of B. eriantha showed potent in vitro antioxidant and anticancer properties. Moreover, the extract showed significant loss of wound healing, thus suggesting that it could prevent a possible metastasis. Hence, B. eriantha could be explored as a potential anticancer plant with antimetastatic properties.

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Abbreviations

DMEM:

Dulbecco’s modified Eagle’s medium

DPPH:

1,1-diphenyl-2- picrylhydrazyl

FRAP:

Ferric ion reducing antioxidant power assay

HPTLC:

High performance thin layer chromatography

LC-MS:

Liquid chromatography–mass spectrometry

MTT:

(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

PBS:

Phosphate buffered saline

TFC:

Total flavonoid content

TPC:

Total phenolic content

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Acknowledgments

We would like to thank Dr. Umesh Jagtap, Department of Biotechnology, Shivaji University, Kolhapur, India for his support.

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  1. Department of Biotechnology and Bioinformatics, Padmashree Dr. D.Y. Patil University, Sect-15/50 C.B. D. Belapur, Navi Mumbai, 400 614, India, MS

    Mohini Gore & N. S. Desai

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Correspondence to N. S. Desai.

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Gore, M., Desai, N.S. Characterization of phytochemicals and evaluation of anti-cancer potential of Blumea eriantha DC.. Physiol Mol Biol Plants 20, 475–486 (2014). https://doi.org/10.1007/s12298-014-0246-2

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  • DOI: https://doi.org/10.1007/s12298-014-0246-2

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