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Impacts of methyl jasmonate and phenyl acetic acid on biomass accumulation and antioxidant potential in adventitious roots of Ajuga bracteosa Wall ex Benth., a high valued endangered medicinal plant

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Abstract

Ajuga bracteosa is a medicinally important plant globally used in the folk medicine against many serious ailments. In the present study, effects of two significant elicitors, methyl jasmonate (Me-J) and phenyl acetic acid (PAA) were studied on growth parameters, secondary metabolites production, and antioxidant potential in adventitious root suspension cultures of A. bracteosa. The results showed a substantial increase in biomass accumulation, exhibiting longer log phases of cultures growth in response to elicitor treatments, in comparison to control. Maximum dry biomass formation (8.88 DW g/L) was recorded on 32nd day in log phase of culture when  0.6 mg/L Me-J was applied; however, PAA at 1.2 mg/L produced maximum biomass (8.24 DW g/L) on day 40 of culture.  Furthermore, we observed the elicitors-induced enhancement in phenolic content (total phenolic content), flavonoid content (total flavonoid content) and antioxidant activity (free radical scavenging activity) in root suspension cultures of A. bracteosa. Application of 0.6 mg/L and 1.2 mg/L of Me-J, root cultures accumulated higher TPC levels (3.6 mg GAE/g DW) and (3.7 mg GAE/g DW) in the log phase and stationary phase, respectively, while 2.5 mg/L Me-J produced lower levels (1.4 mg GAE/g DW) in stationary phase of growth stages. Moreover, TFC and FRSA values were found in correspondence to TPC values in the respective growth phases at the similar elicitor treatment. Thus, a feasible protocol for establishment of adventitious roots in A. bracteosa was developed and enhancement in biomass and metabolite content in adventitious root was promoted through elicitation.

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References

  • Abbasi BH, Khan MA, Mahmood T, Ahmad M, Chaudhary MF, Khan MA (2010) Shoot regeneration and free-radical scavenging activity in Silybum marianum L. Plant Cell Tissue Organ Cult (PCTOC) 101:371–376. doi:10.1007/s11240-010-9692-x

    Article  CAS  Google Scholar 

  • Abbasi BH, Ali H, Yücesan B, Saeed S, Rehman K, Khan MA (2016) Evaluation of biochemical markers during somatic embryogenesis in Silybum marianum L. 3 Biotech 6:1–8. doi:10.1007/s13205-016-0366-1

    Article  Google Scholar 

  • Ali M, Hahn E-J, Paek K-Y (2007) Methyl Jasmonate and salicylic acid induced oxidative stress and accumulation of phenolics in Panax ginseng bioreactor root suspension cultures. Molecules 12:607

    Article  CAS  PubMed  Google Scholar 

  • Arfan M, Khan GA, Ahmad N (1996) Steroids and terpenoids of the genus Ajuga. J Chem Soc Pak 18:170–174

    CAS  Google Scholar 

  • Bathoju G, Giri A (2012) Production of medicinally important secondary metabolites (stigmasterol and hecogenin) from root cultures of Chlorophytum borivilianum (Safed musli). Recent Res Sci Technol 4:45–48

    CAS  Google Scholar 

  • Chang C-C, Yang M-H, Wen H-M, Chern J-C (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10(3):178–182

  • Chopra RN, Chopra IC, Nayar SL (1956) Glossary of Indian medicinal plants. Council of Scientific and Industrial Research, New Delhi

    Google Scholar 

  • De Klerk G-J, Brugge JT, Marinova S (1997) Effectiveness of indoleacetic acid, indolebutyric acid and naphthaleneacetic acid during adventitious root formation in vitro in Malus ‘Jork 9’. Plant Cell Tissue Organ Cult 49:39–44. doi:10.1023/a:1005850222973

    Article  Google Scholar 

  • Dinan L (2001) Phytoecdysteroids: biological aspects. Phytochemistry 57:325–339. doi:10.1016/S0031-9422(01)00078-4

    Article  CAS  PubMed  Google Scholar 

  • Dixon RA, Paiva NL (1995) Stress-induced phenylpropanoid metabolism. Plant Cell 7:1085

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Dunlap JR, Kresovich S, McGee RE (1986) The effect of salt concentration on auxin stability in culture media. Plant Physiol 81:934–936

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gapper C, Dolan L (2006) Control of plant development by reactive oxygen species. Plant Physiol 141:341–345

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gautam R, Jachak SM, Saklani A (2011) Anti-inflammatory effect of Ajuga bracteosa Wall. ex Benth. mediated through cyclooxygenase (COX) inhibition. J Ethnopharmacol 133:928–930

    Article  PubMed  Google Scholar 

  • Gundlach H, Muller MJ, Kutchan TM, Zenk MH (1992) Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proc Natl Acad Sci USA 89:2389–2393

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Heller W, Forkmann G (1988) Biosynthesis. In: Harborne JB, Mabry TJ, Mabry H (eds) The flavonoids. Springer, pp 399–425

  • Jalalpour Z, Shabani L, Afghani L, Sharifi-Tehrani M, Amini S-A (2014) Stimulatory effect of Methyl Jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew. Turk J Biol 38:76–82

    Article  CAS  Google Scholar 

  • Jan M, Singh S, Kaloo ZA, Maqbool F (2014) Callus induction and multiple shoot regeneration in Ajuga bracteosa Wall. ex Benth.—an important medicinal plant growing in Kashmir Himalaya. J Sci Innov Res 3:319–324

    Google Scholar 

  • Kayani WK, Rani R, Ihsan-ul H, Mirza B (2014) Seasonal and geographical impact on the morphology and 20-hydroxyecdysone content in different tissue types of wild Ajuga bracteosa Wall. ex Benth. Steroids 87:12–20. doi:10.1016/j.steroids.2014.04.017

    Article  CAS  PubMed  Google Scholar 

  • Khan MA, Abbasi BH, Ahmed N, Ali H (2013) Effects of light regimes on in vitro seed germination and silymarin content in Silybum marianum. Ind Crops Prod 46:105–110

    Article  CAS  Google Scholar 

  • Khan MA, Abbasi BH, Ali H, Ali M, Adil M, Hussain I (2015a) Temporal variations in metabolite profiles at different growth phases during somatic embryogenesis of Silybum marianum L. Plant Cell Tissue Organ Cult (PCTOC) 120:127–139

    Article  CAS  Google Scholar 

  • Khan MA, Abbasi BH, Shah NA, Yücesan B, Ali H (2015b) Analysis of metabolic variations throughout growth and development of adventitious roots in Silybum marianum L. (Milk thistle), a medicinal plant. Plant Cell Tissue Organ Cult (PCTOC) 123:501–510. doi:10.1007/s11240-015-0854-8

    Article  Google Scholar 

  • Lee YS, Yang T-J, Park SU, Baek JH, Wu SQ, Lim K-B (2011) Induction and proliferation of adventitious roots from ‘Aloe vera’ leaf tissues for ‘in vitro’ production of aloe-emodin. Plant Omics 4:190

    CAS  Google Scholar 

  • Lister CE, Lancaster JE, Walker JR (1996) Developmental changes in enzymes of flavonoid biosynthesis in the skins of red and green apple cultivars. J Sci Food Agric 71:313–320

    Article  CAS  Google Scholar 

  • Liszkay A, van der Zalm E, Schopfer P (2004) Production of reactive oxygen intermediates (O2 −, H2O2, and ˙OH) by maize roots and their role in wall loosening and elongation growth. Plant Physiol 136:3114–3123. doi:10.1104/pp.104.044784

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Misico RI, Nicotra VE, Oberti JC, Barboza G, Gil RR, Burton G (2011) Withanolides and related steroids. In: Progress in the chemistry of organic natural products, vol 94. Springer, pp 127–229

  • Mulabagal V, Tsay H-S (2004) Plant cell cultures-an alternative and efficient source for the production of biologically important secondary metabolites. Int J Appl Sci Eng 2:29–48

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Paek K-Y, Hahn E-J, Son S-H (2001) Application of bioreactors for large-scale micropropagation systems of plants. In Vitro Cell Dev Biol Plant 37:149–157

    Article  CAS  Google Scholar 

  • Pala A et al (2011) Ajuga bracteosa wall: a review on its ethnopharmacological and phytochemical studies. Der Pharm Sin 2:1–10

    Google Scholar 

  • Peeters AJ, Gerards W, Barendse GW, Wullems GJ (1991) In vitro flower bud formation in tobacco: interaction of hormones. Plant Physiol 97:402–408

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Thanh N, Murthy H, Yu K, Hahn E, Paek K (2005) Methyl Jasmonate elicitation enhanced synthesis of ginsenoside by cell suspension cultures of Panax ginseng in 5-l balloon type bubble bioreactors. Appl Microbiol Biotechnol 67:197–201

    Article  CAS  PubMed  Google Scholar 

  • Velioglu Y, Mazza G, Gao L, Oomah B (1998) Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 46:4113–4117

    Article  CAS  Google Scholar 

  • Yan Y-H et al (2014) Effect of naphthalene acetic acid on adventitious root development and associated physiological changes in stem cutting of Hemarthria compressa. PLoS ONE 9:e90700. doi:10.1371/journal.pone.0090700

    Article  PubMed  PubMed Central  Google Scholar 

  • Yang L, Stöckigt J (2010) Trends for diverse production strategies of plant medicinal alkaloids. Nat Prod Rep 27:1469–1479

    Article  CAS  PubMed  Google Scholar 

  • Zhang J, Gao W-Y, Wang J, Li X-L (2011) Effects of explant types and media salt strength on growth and secondary metabolite accumulation in adventitious roots of Periploca sepium Bunge. Acta Physiol Plant 33:2447–2452. doi:10.1007/s11738-011-0785-x

    Article  CAS  Google Scholar 

  • Zhang J, Gao W-Y, Wang J, Li X (2012) Effects of sucrose concentration and exogenous hormones on growth and periplocin accumulation in adventitious roots of Periploca sepium Bunge. Acta Physiol Plant 34:1345–1351

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Biotechnology Program, Department of Environmental Sciences, COMSATS Institute of Information Technology (CIIT), Abbottabad, Pakistan

    Sabahat Saeed

  2. Department of Biotechnology, Bacha Khan University Charsadda, Charsadda, KP, Pakistan

    Huma Ali

  3. Department of Biotechnology, University of Malakand, Chakdara, Dir Lower, Pakistan

    Tariq Khan

  4. Department of Botany, The University of Poonch Rawalakot, Rawalakot, Azad Kashmir, Pakistan

    Waqas Kayani

  5. Department of Biotechnology, Faculty of Chemical and Life Sciences, Abdul Wali Khan University Mardan (AWKUM), Mardan, 23200, KP, Pakistan

    Mubarak Ali Khan

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  1. Sabahat Saeed
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  2. Huma Ali
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  3. Tariq Khan
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  4. Waqas Kayani
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Correspondence to Mubarak Ali Khan.

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Saeed, S., Ali, H., Khan, T. et al. Impacts of methyl jasmonate and phenyl acetic acid on biomass accumulation and antioxidant potential in adventitious roots of Ajuga bracteosa Wall ex Benth., a high valued endangered medicinal plant. Physiol Mol Biol Plants 23, 229–237 (2017). https://doi.org/10.1007/s12298-016-0406-7

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  • DOI: https://doi.org/10.1007/s12298-016-0406-7

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