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Plant growth promoting effect of Bacillus amyloliquefaciens H-2-5 on crop plants and influence on physiological changes in soybean under soil salinity

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Abstract

This study was aimed to identify plant growth-promoting bacterial isolates from soil samples and to investigate their ability to improve plant growth and salt tolerance by analysing phytohormones production and phosphate solubilisation. Among the four tested bacterial isolates (I-2-1, H-1-4, H-2-3, and H-2-5), H-2-5 was able to enhance the growth of Chinese cabbage, radish, tomato, and mustard plants. The isolated bacterium H-2-5 was identified as Bacillus amyloliquefaciens H-2-5 based on 16S rDNA sequence and phylogenetic analysis. The secretion of gibberellins (GA4, GA8, GA9, GA19, and GA20) from B. amyloliquefaciens H-2-5 and their phosphate solubilisation ability may contribute to enhance plant growth. In addition, the H-2-5-mediated mitigation of short term salt stress was tested on soybean plants that were affected by sodium chloride. Abscisic acid (ABA) produced by the H-2-5 bacterium suppressed the NaCl-induced stress effects in soybean by enhancing plant growth and GA4 content, and by lowering the concentration of ABA, salicylic acid, jasmonic acid, and proline. These results suggest that GAs, ABA production, and the phosphate solubilisation capacity of B. amyloliquefaciens H-2-5 are important stimulators that promote plant growth through their interaction and also to improve plant growth by physiological changes in soybean at saline soil.

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Acknowledgement

This research was supported by Agenda Program (Project No. PJ01228603) through the Rural Development Administration, Republic of Korea.

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Author notes

  1. Min-Ji Kim and Ramalingam Radhakrishnan have contributed equally to this work.

Authors and Affiliations

  1. School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Republic of Korea

    Min-Ji Kim, Sang-Mo Kang, Eun-Ju Jeong & In-Jung Lee

  2. School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, 712-749, Republic of Korea

    Ramalingam Radhakrishnan

  3. National University Marine Microorganism Team, National Marine Biodiversity Institute of Korea, Seocheon, 33662, Republic of Korea

    Young-Hyun You

  4. School of Life Sciences, Kyungpook National University, Daegu, Republic of Korea

    Jong-Guk Kim

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  1. Min-Ji Kim
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Correspondence to In-Jung Lee.

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Kim, MJ., Radhakrishnan, R., Kang, SM. et al. Plant growth promoting effect of Bacillus amyloliquefaciens H-2-5 on crop plants and influence on physiological changes in soybean under soil salinity. Physiol Mol Biol Plants 23, 571–580 (2017). https://doi.org/10.1007/s12298-017-0449-4

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  • DOI: https://doi.org/10.1007/s12298-017-0449-4

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