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In vitro propagation of Cymbidium goeringii Reichenbach fil. through direct adventitious shoot regeneration

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Abstract

The influence of 2,4-dichlorophenoxyacetic acid (2,4-D), benzyladenine (BA), and thidiazuron (TDZ) on direct rhizome induction and shoot formation from rhizome explants of Cymbidium goeringii was explored. Rhizome segments obtained from in vitro seed cultures of C. goeringii were placed on Murashige and Skoog (MS) medium incorporated with 5, 10, 20, or 40 µM 2,4-D and 1, 2, 4, or 8 µM BA or TDZ alone or in combination with 20 µM 2,4-D. The explants developed only rhizomes on MS medium with or without 2,4-D. The highest percent of rhizome formation (100%) was obtained on MS medium incorporated with 20 μM of 2,4-D. The morphology and number of rhizomes varied with the level of 2,4-D in the medium. Direct adventitious shoot formation was achieved on medium incorporated with BA or TDZ. The adventitious shoots produced per explant significantly increased with the supplementation of 2,4-D to cytokinin-containing medium. The highest mean of 21.8 ± 1.8 shoot buds per rhizome segment was obtained in medium fortified with 20 μM 2,4-D and 2 μM TDZ. The greatest percent of root induction (100%) and the mean of 5.3 ± 1.1 roots per shoot were achieved on ½ MS medium incorporated with 2 μM of α-naphthaleneacetic acid. About 97% of the in vitro-produced plantlets acclimatized in the greenhouse. An efficient in vitro propagation protocol was thus developed for C. goeringii using rhizome explants.

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Acknowledgements

This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea. Project No: 315025-3-3-HD040. This article was supported by the KU Research Professor Program of Konkuk University.

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Authors and Affiliations

  1. Department of Bioresource Engineering, Sejong University, Seoul, 143-747, South Korea

    Han Yong Park

  2. Babo Orchid Farm, Namyangju-si, Gyeonggi-do, 472-831, South Korea

    Kyung Won Kang

  3. Department of Bioresources and Food Science, Konkuk University, Seoul, 143-701, South Korea

    Doo Hwan Kim & Iyyakkannu Sivanesan

Authors
  1. Han Yong Park
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  2. Kyung Won Kang
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  3. Doo Hwan Kim
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  4. Iyyakkannu Sivanesan
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Contributions

Han Yong Park and Iyyakkannu Sivanesan designed the experiment, analyzed the data and prepared the manuscript. Kyung Won kang and Doo Hwan Kim edited the manuscript.

Corresponding author

Correspondence to Iyyakkannu Sivanesan.

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Park, H.Y., Kang, K.W., Kim, D.H. et al. In vitro propagation of Cymbidium goeringii Reichenbach fil. through direct adventitious shoot regeneration. Physiol Mol Biol Plants 24, 307–313 (2018). https://doi.org/10.1007/s12298-017-0503-2

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  • DOI: https://doi.org/10.1007/s12298-017-0503-2

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