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RAPD, ISSR, and SCoT markers based genetic stability assessment of micropropagated Dendrobium fimbriatum Lindl. var. oculatum Hk. f.- an important endangered orchid

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Abstract

Dendrobium fimbriatum is an ornamental and medicinal orchid listed in the Red data book of IUCN. Phytohormones’ effect on the in vitro regeneration of the orchid was studied using Mitra medium supplemented with different growth regulators. KN produced effective shoot formation when present alone or in combination with IBA or NAA. The shooting was gradually increased when KN concentration was increased from 0.8 to 4.8 mg L−1, but the opposite response was observed with BAP at higher concentration (4.8 mg L−1). IBA either in combination with BAP or KN promoted effective root development and multiplication. Micropropagated orchids grown in the basal medium devoid of any phytohormone showed 100% monomorphism, while low genetic polymorphism of 1.52% (RAPD—Random Amplification of Polymorphic DNA), 1.19% (ISSR-Inter Simple Sequence Repeat) and 3.97% (SCoT—Start Codon Targeted) was exhibited among the regenerants propagated in the hormone enriched medium. UPGMA (Unweighted pair group method using arithmetic averages) dendrograms showed the grouping of mother plant (MP) with the in vitro regenerants. The principal coordinate analysis (PCoA) further confirmed the clustering patterns as determined by the cluster analysis. The study reported for the first time the successful in vitro propagation of Dendrobium fimbriatum and their genetic stability assessment using molecular markers.

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Funding

The research work is funded by Department of Science and Technology (DST), New Delhi, India.

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Authors and Affiliations

  1. Department of Biotechnology, Manipur University, Canchipur, Manipur, 795003, India

    Leimapokpam Tikendra, Angamba Meetei Potshangbam & Potshangbam Nongdam

  2. Department of Life Sciences, Presidency University, Kolkata, 700073, India

    Abhijit Dey

  3. Division of Horticulture, ICAR Research Complex for NEH Region, Lamphelpat, Manipur, 795004, India

    Tongbram Roshini Devi & Manas Ranjan Sahoo

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Contributions

The experiment is conceived and designed by LT and PN. Funding acquisition: PN; Experimentation: LT; Materials and result analysis: LT, TRD and AMP; Writing initial version of the manuscript: LT; Reviewing and editing: AD, MRS and PN; Supervision: PN. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Potshangbam Nongdam.

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Tikendra, L., Potshangbam, A.M., Dey, A. et al. RAPD, ISSR, and SCoT markers based genetic stability assessment of micropropagated Dendrobium fimbriatum Lindl. var. oculatum Hk. f.- an important endangered orchid. Physiol Mol Biol Plants 27, 341–357 (2021). https://doi.org/10.1007/s12298-021-00939-x

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