Abstract
Though the volatile profiles of black pepper have been reported already, the information on terpene synthase family genes is not known. In this study, using a combinatorial approach, the berry hybrid transcriptome assembly of llumina and nanopore sequencing, the entire terpene synthase family responsible for the biosynthesis of the flavor-imparting volatiles in black pepper berries was profiled. The profile shows 98 terpene synthases from various terpene synthesis pathways. Three important monoterpene synthases were also validated by targeted amplification, sequencing and homology modeling. This study provides the first of its kind information on the terpene synthase family profile in Piper nigrum, which is potentially a major step for further characterization of the functional terpene synthase genes in black pepper.
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Acknowledgements
Authors are thankful to Indian Council of Agricultural Research, Ministry of Agriculture and Farmers' Welfare, Govt. of India for providing financial assistance in the form of CABin grant (F. no. Agril. Edn.4-1/2013-A&P) as well as Advanced Super Computing Hub for Omics Knowledge in Agriculture (ASHOKA) facility at Indian Council of Agricultural Research-Indian Agricultural Statistics Research Institute, New Delhi, India. Authors are thankful to Director, ICAR-Indian Agricultural Statistics Research Institute, New Delhi, India and Director, ICAR-Indian Institute of Spice Research, Calicut, India for providing research facility.
Funding
This research was funded by Indian Council of Agricultural Research, Ministry of Agriculture and Farmers' Welfare, Govt. of India for providing financial assistance in the form of CABin grant (F. no. Agril. Edn.4–1/2013-A&P).
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George, J.K., Shelvy, S., Fayad, A.M. et al. De novo transcriptome sequencing assisted identification of terpene synthases from black pepper (Piper nigrum) berry. Physiol Mol Biol Plants 27, 1153–1161 (2021). https://doi.org/10.1007/s12298-021-00986-4
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DOI: https://doi.org/10.1007/s12298-021-00986-4