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Soybean drought-stressed plants impair Anticarsia gemmatalis (Lepidoptera: Erebidae) midgut proteolytic activity and survival

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Abstract

Soybean plants are exposed to multiple stresses during development. Co-occurring stresses can activate common defense pathways on plants, increasing end products concentrations, such as protease inhibitors (PIs). Here, we evaluated the impact of drought stress on soybean defense mechanisms regarding PIs. Furthermore, the midgut proteolytic activity and survival of A. gemmatalis larvae fed with drought-stressed and well-watered plants were assessed. The lipoxygenase activity remained similar on soybean exposed to moderate (−0.6 and − 1.0 MPa) and severe (−1.6 MPa) drought stresses, however were higher than the irrigated control. The PIs activity on soybean was only induced due to herbivory at those exposed to moderate stress (−0.6 and − 1.0 MPa). The total-proteases and trypsin-like reduced activities in the midgut of A. gemmatalis that fed on moderate-stressed plants reflected the effects of the PIs induced after herbivory. Furthermore, A. gemmatalis showed reduced survival on drought-stressed plants over those well-watered. The results add knowledge about factors that limit A. gemmatalis performance.

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Acknowledgments

This research was funded by the “Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)”, “Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)”, “Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)” and “Instituto Nacional de Ciência e Tecnologia em Interações Planta-Praga (INCT-IPP)”. We thank Drs. Ricardo Alcántara-de la Cruz and Wellington Souto Ribeiro for their insightful comments. Authors declare to have not conflict of interests.

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Authors and Affiliations

  1. Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-900, Brazil

    Verônica Aparecida Faustino, Rafael de Almeida Barros, Neilier Rodrigues da Silva Júnior, Samuel Lessa Barbosa, Camilo Elber Vital, Yaremis Beatriz Meriño Cabrera, Humberto Josué de Oliveira Ramos & Maria Goreti de Almeida Oliveira

  2. Departamento de Fitotecnia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-000, Brazil

    Felipe Lopes da Silva

  3. Departamento de Engenharia de Biossistemas, Universidade Federal de São João Del Rei, São João Del Rei, Minas Gerais, 36302-160, Brazil

    Wellington Garcia Campos

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  1. Verônica Aparecida Faustino
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  9. Humberto Josué de Oliveira Ramos
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  10. Maria Goreti de Almeida Oliveira
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Contributions

Maria Goreti de Almeida Oliveira and Humberto Josué de Oliveira Ramos conceived research. Verônica Aparecida Faustino, Felipe Lopes da Silva and Samuel Lessa Barbosa conducted experiments. Verônica Aparecida Faustino, Camilo Elber Vital and Rafael de Almeida Barros analyzed data and conducted statistical analyzes. Verônica Aparecida Faustino, Camilo Elber Vital, Rafael de Almeida Barros and Neilier Rodrigues da Silva Júnior wrote the manuscript. Maria Goreti de Almeida Oliveira secured funding. All authors read and approved the manuscript.

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Correspondence to Humberto Josué de Oliveira Ramos.

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Faustino, V.A., de Almeida Barros, R., da Silva Júnior, N.R. et al. Soybean drought-stressed plants impair Anticarsia gemmatalis (Lepidoptera: Erebidae) midgut proteolytic activity and survival. Phytoparasitica 49, 491–500 (2021). https://doi.org/10.1007/s12600-020-00873-w

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