Skip to main content
SpringerLink
Log in

Comparative Evaluation of Oral Administration of Probiotic Lactobacilli-fermented Milks on Macrophage Function

  • Published:
Probiotics and Antimicrobial Proteins Aims and scope Submit manuscript

Abstract

Six strains of lactobacilli belonging to three species (Lactobacillus casei, Lactobacillus acidophilus and Lactobacillus helveticus) were evaluated for probiotic attributes viz. acid tolerance, bile tolerance and cell surface hydrophobicity. All the six strains exhibited probiotic attributes with considerable degree of variation. Three Lactobacillus strains selected on the basis of probiotic attributes were used for preparing three different fermented milks. In order to evaluate the effect of feeding these probiotic fermented milks on macrophage cell function, an in-vivo trial was conducted in mice for a period of 2, 5 and 8 days. The control group of mice was fed with skim milk. The phagocytic activity of macrophages increased significantly (P < 0.05) on feeding fermented milk prepared using L. acidophilus, L. casei and L. helveticus as compared to milk group (control) on 2nd, 5th and 8th day of feeding, respectively. Likewise, the release of β-glucuronidase and β-galactosidase from peritoneal macrophages increased significantly (P < 0.05) on 2nd, 5th and 8th day of feeding as compared to their respective control group (milk). The results thus depict that feeding of probiotic fermented milk enhances phagocytic activity of the macrophages.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Schaffsma G (1996) State of the art concerning probiotic strains in milk products. IDF Nutr Newsl 5:23–24

    Google Scholar 

  2. Reid G, Jass J, Sebulsky MT, McCormick JK (2003) Potential uses of probiotics inclinical practice. Clin Microbiol Rev 16:658–672

    Article  Google Scholar 

  3. Gill HS, Guarner F (2004) Probiotics and human health: a clinical perspective. Postgrad Med J 80:516–526

    Article  CAS  Google Scholar 

  4. Mercenier A, Pavan S, Pot B (2003) Probiotics as biotherapeutic agents: present knowledge and future prospects. Curr Pharm Des 8:99–110

    Google Scholar 

  5. Shanahan F (2003) Probiotics in inflammatory bowel disease: therapeutic rationale and role. Adv Drug Deliv Rev 56:809–818

    Article  Google Scholar 

  6. Stanton C, Gardiner G, Meehan H, Collins K, Fitzgerald G, Lynch PB, Ross RP (2001) Market potential for probiotics. Am J Clin Nutr 73(Suppl.):476S–483S

    Google Scholar 

  7. Naidu AS et al (1999) Probiotic spectra of lactic acid bacteria. Crit Rev Food Sci Nutr 39(1):13–126

    Article  CAS  Google Scholar 

  8. Galdenao MC, Perdigon G (2006) The probiotic bacterium Lactobacillus casei induces activation of the gut immune system through innate immunity. Clin Vaccine Immunol 13:219–226

    Article  Google Scholar 

  9. Dunne C, Mahony LO, Murphy L, Thorton G, Morrissey D, Halloran SO, Feeney M, Flynn S, Fitzgerald G, Daly C, Kiely B, GCO’ Sullivan, Shanahan F, Collins JK (2001) In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. Am J Clin Nutr 73:386S–392S

    CAS  Google Scholar 

  10. Tuomola E, Crittenden R, Playne M, Isolauri E, Salminen S (2001) Quality assurance criteria for probiotic bacteria. Am J Clin Nutr 73:393S–398S

    CAS  Google Scholar 

  11. Clark PA, Cotton LN, Martin JH (1997) Selection of bifidobacteria for use as delivery adjuncts in cultured dairy foods. II. Tolerance to stimulated pH of human stomachs. Cul Dairy Prod J 28(4):11–14

    Google Scholar 

  12. Gilliland SE, Staley TE, Bush LJ (1984) Importance of bile tolerance of Lactobacillus acidophilus used as dietary adjunct. J Dairy Sci 67:3045–3051

    Article  CAS  Google Scholar 

  13. Rosenberg M, Gutnick D, Rosenberg E (1980) Adherence of bacteria to hydrocarbons: a simple method for measuring cell-surface hydrophobicity. FEMS Microbiol Lett 9:29–33

    Article  CAS  Google Scholar 

  14. Stossel TP (1980) Phagocytosis. In: Rose N, Friedman H (eds) Manual of clinical immunology, 2nd edn. Am Soc Microbiol 313

  15. Conchie J, Findlay J, Levy J (1959) Mammalian glycosidase distribution in the body. Biochem J 71:318–325

    CAS  Google Scholar 

  16. Hay FC, Westwood OMR (2002) Phagocytosis, complement, antibody-dependent cytotoxicity. In: Hay FC and Westwood OMR (eds) Practical immunology. Blackwell Science, London, pp 203–205

  17. Martini MC, Bolweg GL, Levitt MD, Savaiano DA (1987) Lactose digestion by yoghurt β-galactosidase. Influence of pH and microbial cell integrity. Am J Clin Nutr 45:432–437

    CAS  Google Scholar 

  18. Jacobsen CN, Rosenfeldt NV, Hayford AE, Moller PL, Michaeken KF, Paerragaard A, Sandstrom B, Tvede M, Jakobsen M (1999) Screening of probiotic activities of forty seven strains of Lactobacillus spp. by in vitro techniques and evaluation of colonization ability of five selected strains in human. Appl Environ Microbiol 65:4949–4956

    CAS  Google Scholar 

  19. Mishra V, Prasad DN (2005) Application of in vitro methods for selection of Lactobacillus casei strains as potential probiotics. Int J Food Microbiol 103:109–115

    Article  Google Scholar 

  20. Morata De Ambrosini V, Gonzalez S, Pesce De Ruiz Holgando A, Oliver G (1998) Study of morphology of cell walls of some strains of lactic acid bacteria and related species. J Food Prot 61:557–562

    CAS  Google Scholar 

  21. Perdigon D, de Macias Alvarez S, Oliver G, de Ruiz Holgado AP (1986) Lactobacilli administered orally induce release of enzyme from peritoneal macrophages in mice. Milchwissenschaft 41:344–348

    CAS  Google Scholar 

  22. Perdigon D, de Macias Alvarez S, Oliver G, de Ruiz Holgado AP (1986) Effect of perorally administered lactobacilli on macrophage activation in mice. Infect Immunol 53:404–410

    CAS  Google Scholar 

  23. Perdigon G, de Macias Alvarez S (1992) Probiotics and the immune state. In: Fuller R (ed) Probiotics. Chapman & Hall, London, pp 146–176

    Google Scholar 

  24. Kapila S, Sinha PR, Singh S (2007) Influence of feeding fermented milk and non-fermented milk containing Lactobacillus casei on immune response in mice. Food Agril Immunol 18:75–82

    Article  CAS  Google Scholar 

  25. Schynder J, Bagglioini M (1978) Secretion of lysosomal hydrolases by stimulated and non stimulated macrophages. J Exp Med 148:435–450

    Article  Google Scholar 

Download references

Acknowledgments

The authors acknowledge Dr. V. K. Kansal, ExPrincipal Scientist, NDRI for providing L. acidophilus VK2 strain. The financial aid to carry out this study by Department of Biotechnology (DBT), Government of India, is fully acknowledged.

Author information

Authors and Affiliations

  1. NDRI, Karnal, Haryana, India

    Rajeev Kapila, Suman Kapila, Meena Kapasiya, Divya Pandey, Ajay Dang & Vamshi Saliganti

Authors
  1. Rajeev Kapila
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Suman Kapila
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Meena Kapasiya
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Divya Pandey
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ajay Dang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Vamshi Saliganti
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Suman Kapila.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kapila, R., Kapila, S., Kapasiya, M. et al. Comparative Evaluation of Oral Administration of Probiotic Lactobacilli-fermented Milks on Macrophage Function. Probiotics & Antimicro. Prot. 4, 173–179 (2012). https://doi.org/10.1007/s12602-012-9107-x

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12602-012-9107-x

Keywords

Search

Navigation