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Bacillus amyloliquefaciens BLCC1-0238 Can Effectively Improve Laying Performance and Egg Quality Via Enhancing Immunity and Regulating Reproductive Hormones of Laying Hens

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Abstract

In this study, we sought to evaluate the effects of dietary Bacillus amyloliquefaciens (B. amyloliquefaciens) BLCC1-0238 supplementation on laying performance, egg quality, antioxidant enzyme activities, reproductive hormone, and immunity of laying hens. A total of 240 Hy-Line Brown laying hens (28 weeks old) were randomly divided into four groups, and three replicates per group (n = 20 per replicate). The control group was fed a standard basal diet, and the three treatment groups were provided the basal diet supplemented with either 0.01%, 0.03%, or 0.06% B. amyloliquefaciens BLCC1-0238 (2 × 1010 CFU/g), respectively. Hens were allowed 2 weeks to acclimate prior to initiation of the 8-week experiment. It was observed that dietary supplementation with 0.01% or 0.03% B. amyloliquefaciens BLCC1-0238 significantly increased egg production and egg mass. However, no significant differences in feed intake, egg weight, and feed conversion ratio among the four groups were observed. Different levels of B. amyloliquefaciens BLCC1-0238 supplementation also significantly increased egg shell strength and thickness. With respect to the levels of reproductive hormones in the hens, B. amyloliquefaciens BLCC1-0238 supplementation significantly reduced serum adrenal cortical hormone (ACTH) levels, while increasing estradiol (E2) and follicle-stimulating hormone (FSH) secretion in the treatment groups compared to the control group. Relative to the control group, supplementation with 0.03% and 0.06% B. amyloliquefaciens BLCC1-0238 was observed to significantly increase serum glutathione S-transferase (GST) concentration, and supplementation significantly reduced serum IL-1 and IL-6 levels, whereas IL-4 levels increased for all concentrations tested. In conclusion, supplementation of a basal chicken diet with B. amyloliquefaciens BLCC1-0238 can improve laying performance and egg quality through the reduction of stress responses, up-regulation of growth hormones, and supporting immunity in laying hens.

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Abbreviations

ACTH:

adrenal cortical hormone

Bacillus amyloliquefaciens BLCC1-0238:

B. amyloliquefaciens BLCC1-0238

CAT:

catalase

CORT:

corticosterone

E2:

estradiol

FSH:

follicle-stimulating hormone

GR:

glutathione reductase

GSH-Px:

glutathione peroxidase

GST:

glutathione S-transferase

LH:

luteinizing hormone

MDA:

malondialdehyde

P:

progesterone

SOD:

superoxide dismutase

T-AOC:

total antioxidant capacity

TR:

thioredoxin reductase

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Funding

This study was supported by the College Students’ Innovation and Entrepreneurship Training Program of China (201610439269 and 201710439327).

Author information

Author notes

  1. Yufa Zhou and Song Li contributed equally to this work.

Authors and Affiliations

  1. College of Animal Science and Technology, Shanxi Agricultural University, Mingxian South Road 1, Taigu, 030801, China

    Yufa Zhou & Quanhai Pang

  2. School of Basic Medicine, Taishan Medical University, Tai’an, 271000, China

    Song Li

  3. School of Life Sciences, Taishan Medical University, Changcheng Road 619, Tai’an, 271018, China

    Zengmin Miao

Authors
  1. Yufa Zhou
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  2. Song Li
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  3. Quanhai Pang
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  4. Zengmin Miao
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Corresponding authors

Correspondence to Quanhai Pang or Zengmin Miao.

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Ethics Statement

All procedures used in this study were approved by the Ethics Committee for Animal Testing of Taishan Medical University, China (approval no. 20170316, 9 March 2017).

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The authors declare that they have no conflict of interest.

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Zhou, Y., Li, S., Pang, Q. et al. Bacillus amyloliquefaciens BLCC1-0238 Can Effectively Improve Laying Performance and Egg Quality Via Enhancing Immunity and Regulating Reproductive Hormones of Laying Hens. Probiotics & Antimicro. Prot. 12, 246–252 (2020). https://doi.org/10.1007/s12602-019-9524-1

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