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Delignification of Lignocellulose Biomasses by Alginate–Chitosan Immobilized Laccase Produced from Trametes versicolor IBL-04

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Abstract

An extracellular laccase was produced from a lignin-degrading white-rot fungus Trametes versicolor IBL-04 using the solid-state bioprocessing of corncobs. The enzyme thus produced was effectively immobilized on alginate–chitosan beads. Immobilized laccase furnished the highest immobilization yield (81.43 ± 2.98) under optimized working conditions of 3.0% (w/v) sodium alginate concentration, 2.4% CaCl2, 0.25% chitosan concentration following an immobilization time of 5 h. The immobilization process altered the optimum pH from 5.0 to 4.0 (an acidic shift), whereas the temperature optima were displaced from 40 to 50 °C (towards higher temperature). The Michaelis–Menten constant (K m) and the maximum reaction rate (V max) of the free and immobilized laccase were 0.14 mM, and 780 µmol/mL and 0.10 mM and 814 µmol/mL, respectively. The alginate–chitosan immobilized laccase was more stable and tolerant against the activity inhibition caused by the denaturants tested, concerning its soluble laccase. Delignification results revealed that the enzyme led to a marked reduction in lignin content of all the lignocellulosic wastes with the highest delignification of 78.29% in sugarcane bagasse, followed by wheat bran and corn stover after 15 h. It is demonstrated that the immobilized laccase could be a promising option as a green catalyst for the efficient delignification of lignocellulosic biomass residues.

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Acknowledgements

The authors would like to thank Higher Education Commission (HEC), Islamabad, Pakistan for funding the project focusing on microbial ligninolytic enzymes for industrial applications.

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Authors and Affiliations

  1. Industrial Biotechnology Laboratory, Department of Biochemistry, University of Agriculture Faisalabad, Faisalabad, Pakistan

    Muhammad Asgher, Abdul Wahab & Muhammad Bilal

  2. State Key Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Muhammad Bilal

  3. School of Engineering and Science, Tecnologico de Monterrey, Campus Monterrey, Ave. Eugenio Garza Sada 2501, CP 64849, Monterrey, N.L., Mexico

    Hafiz M. N. Iqbal

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Correspondence to Hafiz M. N. Iqbal.

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Asgher, M., Wahab, A., Bilal, M. et al. Delignification of Lignocellulose Biomasses by Alginate–Chitosan Immobilized Laccase Produced from Trametes versicolor IBL-04. Waste Biomass Valor 9, 2071–2079 (2018). https://doi.org/10.1007/s12649-017-9991-0

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