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Pectinase Production from Schizophyllum commune Through Central Composite Design Using Citrus Waste and Its Immobilization for Industrial Exploitation

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Abstract

Pectinase is an important group of industrial enzymes. Pectinase manufacturing occupies about 10% of the overall enzyme production world over. The aim of this study is to produce pectin lyase from Schizophyllum commune using the mosambi (sweet lime) fruit peels as substrate in solid state fermentation. The cultural parameters optimized through response surface methodology showed maximum pectin lyase production of 480.45 U/mL at initial medium pH of 6, incubation temperature of 35 °C, time period of 1 day, substrate concentration of 15 g and 3 mL of inoculum size. A purification fold of 3.08 with 355 U/mg specific activity and 4.16% yield was obtained after purification. Enzyme immobilization was done by entrapment with sodium alginate and adsorption with chitosan. Chitosan immobilized enzyme exhibited best thermal stability in the range of 45–55 °C and pH 8.0–9.0. Enzyme activity was stimulated in the presence of Ca2+ and Mg2+ while EDTA inhibited the enzyme activity. Chitosan immobilized pectin lyase was stable up to six cycles of reuse. The pH and thermal stability of S. commune pectin lyase makes it an important enzyme for industrial use. The results showed that pectin lyase produced from S. commune has significant potential for applications in the detergent and fruit juice industry. The enzyme produced from citrus agro waste via the proposed optimized biotechnological process can be explored for multiple industrial applications.

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Acknowledgements

The authors would like to acknowledge the Department of Biochemistry and Biotechnology, University of Gujrat (UOG), Pakistan for providing experiment facilities. Very special thanks to Dr. Muhammad Irshad (late) for guidance and valuable suggestion for completion of this project form UOG. This research was a part of the NRPU funded project from HEC being run at University of Gujrat by Dr. Muhammad Irshad (late).

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Authors and Affiliations

  1. Department of Chemistry, University of Sargodha, Sargodha, 40100, Pakistan

    Tahir Mehmood, Tasmia Saman, Farooq Anwar, Muhammad Salman Ikram & Qudsia Tabassam

  2. Department of Biotechnology, University of Sargodha, Sargodha, 40100, Pakistan

    Muhammad Irfan

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  1. Tahir Mehmood
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  2. Tasmia Saman
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Correspondence to Tahir Mehmood or Muhammad Irfan.

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Mehmood, T., Saman, T., Irfan, M. et al. Pectinase Production from Schizophyllum commune Through Central Composite Design Using Citrus Waste and Its Immobilization for Industrial Exploitation. Waste Biomass Valor 10, 2527–2536 (2019). https://doi.org/10.1007/s12649-018-0279-9

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