Abstract
We document isolation and characterization of 13 tri- and tetranucleotide microsatellite DNA markers in brook trout (Salvelinus fontinalis). These markers displayed moderate to high levels of allelic diversity (averaging 20.5 alleles/locus) and heterozygosity (averaging 53.5%) in a range-wide survey of more than 13,000 fish. A comparison of two geographically proximal populations located on opposite sides of the eastern continental divide in Maryland, USA, found no deviations from Hardy–Weinberg equilibrium and minimal linkage disequilibrium. Microsatellite markers developed for S. fontinalis yielded sufficient genetic diversity to: (1) produce unique multilocus genotypes; (2) elucidate phylogeographic structure; and (3) provide unique demographic perspectives of population sizes and historical demographics. This suite of markers also provided considerable cross-species amplification utility among related salmonids.
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Acknowledgments
This research was funded by the U.S. Geological Survey’s (USGS) and National Park Service’s (NPS) joint Natural Resource Preservation Program (NRPP) with additional funding provided by the USGS Leetown Science Center. The authors wish to thank Roseanna Coleman, Shannon Julian, and Colleen Young for critical laboratory assistance. This article is in partial fulfillment of a final report for NRPP. The authors wish to thank Diane Pavek (Research and T&E Coordinator, National Capital Region, National Park Service) for coordination of this research project. This article also serves as contribution 1676 of the USGS Great Lakes Science Center.
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King, T.L., Lubinski, B.A., Burnham-Curtis, M.K. et al. Tools for the management and conservation of genetic diversity in brook trout (Salvelinus fontinalis): tri- and tetranucleotide microsatellite markers for the assessment of genetic diversity, phylogeography, and historical demographics. Conservation Genet Resour 4, 539–543 (2012). https://doi.org/10.1007/s12686-012-9603-z
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DOI: https://doi.org/10.1007/s12686-012-9603-z