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Study of fertility restoration and genetic diversity of drought-tolerant breeding lines for hybrid rice (Oryza sativa L.) development

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Abstract

The identification of fertility restoration and genetic diversity of drought-tolerant breeding lines will be useful for the development of promising drought-tolerant rice hybrids. The extent of genetic variation of 204 drought-tolerant breeding lines based on the coefficient of parentage (COP) and test crossing with IR 58025A is studied. The linked molecular markers to Rf genes were screened on identified maintainers and restorers. The average value of COP for 204 drought-tolerant breeding lines was 0.21, which indicates genetic divergence in breeding lines. Four major groups comprising of 81 IR 64 type, 37 Apo type, 28 Vandana type, 6 WAB type and 52 others were identified. Out of 204 breeding lines, 37 (18.14%) maintainers, 55 (26.96%) partial maintainers, 63 (30.88%) partial restorers and 49 (24.02%) restorers were identified. Rf genes linked molecular markers RM7466 and RM443 on chr. 1; RM6344 on chr. 7 and RM6100, RM1108, RM216, RM171 and RM244 on chr. 10 were found associated with restorers and maintainers. These Rf genes linked molecular markers might be furthermore used in marker-assisted restorer selection in cytoplasmic male sterile-WA system from large source nurseries to avoid usual test crosses in the hybrid rice development programme. The identified restorer and maintainers may be used for the development of high-yielding drought-tolerant rice hybrids.

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Abbreviations

WA:

Wild-abortive

CMS:

Cytoplasmic male sterile

Rf :

Restorer fertility gene

QTL:

Quantitative trait loci

SSR:

Simple sequence repeat

COP:

Coefficient of parentage

SMA:

Single marker analysis

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Acknowledgements

We are thankful to Barwale Foundation, Hyderabad for providing all the facilities for the execution of this research work and all the encouragement and support.

Funding

This study was funded by Barwale Foundation, Hyderabad, India.

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Author notes

  1. Rahul Priyadarshi

    Present address: International Rice Research Institute (IRRI)-India Office, Guwahati, 502324, Assam, India

Authors and Affiliations

  1. Barwale Foundation, Research and Training Centre, Hyderabad, India

    Rahul Priyadarshi, Akhilesh Kumar Singh & Abhinav Jain

  2. International Rice Research Institute, South Asia Hub, ICRISAT, Patancheru, Hyderabad, India

    Abhinav Jain

  3. ICAR-Central Soil Salinity Research Institute, Karnal, Haryana, India

    Vijayata Singh

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  1. Vijayata Singh
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  2. Rahul Priyadarshi
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  4. Abhinav Jain
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Contributions

RP, VS and AKS were designed and conduct the experiment, recording of data and reviewed the manuscript. RP performed the molecular work, analysis of data and wrote the manuscript. AJ assisted to conduct experimental work, drafting and reviewing of the manuscript. All authors approved the final version of the manuscript.

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Correspondence to Rahul Priyadarshi.

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Singh, V., Priyadarshi, R., Singh, A.K. et al. Study of fertility restoration and genetic diversity of drought-tolerant breeding lines for hybrid rice (Oryza sativa L.) development. J. Crop Sci. Biotechnol. 25, 51–61 (2022). https://doi.org/10.1007/s12892-021-00112-6

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