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Effect of naturally occurring antimicrobials and chemical preservatives on the growth of Aspergillus Parasiticus

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Abstract

Effect of water activity (aw, 0.99), pH (4.5) and their interaction on the growth inhibition of Aspergillus parasiticus was studied on potato dextrose agar (PDA) using various antimicrobial agents (citral, carvacrol, eugenol, cineole, thymol guaiacol, vanillin, anethol, potassium sorbate and sorbic acid). The results demonstrate that colony diameter (mm) exhibited a constant increase with time (zero order kinetics) for all antimicrobials evaluated. Eugenol and sorbic acid inhibited the test fungi at 300 and 600 ppm, respectively. Radial growth rate (RGR) of A. parasiticus was significantly (p < 0.05) different among different antimicrobials as well as the concentrations tested. However, this difference was not observed with higher concentration of citral, eugenol, vanillin and sorbic acid. Among the antimicrobials evaluated potassium sorbate, cineole, anethol and guaiacol were least effective. Thymol, eugenol and carvacrol were more effective in inhibiting A. parasiticus even with low concentration (150 ppm) as their mean RGR was zero even after 20 days of incubation (pH 4.5).

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References

  • Baratta MT, Dorman HJD, Deans SG, Biondi DM, Ruberto G (1998) Chemical composition, antibacterial and antioxidative activity of laurel, sage, rosemary, oregano and coriander essential oils. J Essent Oil Res 10:618–627

    CAS  Google Scholar 

  • Beuchat LR (1994) Antimicrobial properties of spices and their essential oils. In: Dillon VM, Board RG (eds) Natural Antimicrobial Systems and Food Preservation. CAB Intl, Wallingford, pp 167–179

    Google Scholar 

  • Brancato FP, Golding NS (1953) The diameter of the mold colony as a reliable measure of growth. Mycologia 45:848–864

    Google Scholar 

  • Castanon X, Argaiz A, Lopez-Malo A (1999) Effect of storage temperature on the microbial and color stability of banana purees prepared with the addition of vanillin or potassium sorbate. Food Sci Technol Int 5:56–60

    Article  Google Scholar 

  • Cerruti P, Alzamora SM, Chirife J (1990) A multi-parameter approach to control the growth of Saccaromyces cerevisiae in laboratory media. J Food Sci 55:837–840

    Article  Google Scholar 

  • Chen CS (1989) Water activity— concentration molds for solutions of sugars, salts and acids. J Food Sci 54(5):1318–1321

    Article  CAS  Google Scholar 

  • Chung YM, Lee JS (1982) Potassium sorbate inhibition of microorganisms isolated from seafood. J Food Prot 45:1310

    CAS  Google Scholar 

  • Conner DE, Beuchat LR (1984a) Effects of essential oils from plants on growth of food spoilage yeasts. J Food Sci 49:429–434

    Article  Google Scholar 

  • Conner DE, Beuchat LR (1984b) Sensitivity of heat-stressed yeasts to essential oils of plants. Appl Environ Microbiol 47:229–233

    CAS  Google Scholar 

  • Davidson PM (2001) Chemical preservatives and naturally antimicrobial compounds. In: Doyle MP, Beuchat LR, Montville TJ (eds) Food Microbiology. Fundamentals and Frontiers, 2nd edn. ASM Press, Washington, pp 593–628

    Google Scholar 

  • Davidson PM, Naidu AS (2000) Phyto-phenols. In: Naidu AS (ed) Natural Food Antimicrobial Systems. CRC Press, Boca Raton, pp 265–294

    Google Scholar 

  • Dorman HJD, Deans SG (2000) Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol 88:308–316

    Article  CAS  Google Scholar 

  • Duncan DB (1955) Multiple range and multiple ‘F’ test. Biometrics 11:1–42

    Article  Google Scholar 

  • Elgayyar M, Draughon FA, Golden DA, Mount JR (2001) Antimicrobial activity of essential oils from plants against selected pathogenic and saprophytic microorganisms. J Food Prot 64:1019–1024

    CAS  Google Scholar 

  • Gonzalez HHL, Resnik SL, Vaamonde G (1987) Influence of inoculum size on growth rate and lag phase of fungi isolated from Argentine corn. Int J Food Microbiol 4:111–117

    Article  Google Scholar 

  • Holmquist GU, Walker HW, Stahr HM (1983) Influence of temperature, pH, water activity and antifungal agents on growth of Aspergillus flavus and A. parasiticus. J Food Sci 48:778–782

    Article  CAS  Google Scholar 

  • Hope R, Jestoi M, Magan N (2003) Multitarget environmental approach for control of growth and toxin production by Fusarium culmorum using essential oils and antioxidants. In: Credland PE, Armitage DM, Bell CH, Cogan PM, Highley E (eds) Advances in Stored Product Protection. CABI Publishing, Cambridge, pp 486–492

    Google Scholar 

  • Horner KJ, Anagnostopoulos GD (1973) Combined effect of water activity, pH and temperature on the growth and spoilage potential of fungi. J Appl Bacteriol 36:427–436

    Article  CAS  Google Scholar 

  • Kabara JJ (1991) Phenols and chelators. In: Russel NJ, Gould GW (eds) Food Preservatives. Blackie & Son, Glasgow, pp 44–71

    Google Scholar 

  • Lindsay RC (1996) Food additives. In: Fennema OR (ed) Food chemistry, 3rd edn. Marcel Dekker, New York, pp 767–823

    Google Scholar 

  • Lis-Balchin M, Deans SG (1997) Bioactivity of selected plant essential oils against Listeria monocytogenes. J Appl Microbiol 82:759–762

    Article  CAS  Google Scholar 

  • Lopez-Malo A, Alzamora SM, Argaiz A (1995) Effect of natural vanillin on germination time and radial growth of moulds in fruit-based agar systems. Food Microbiol 12:213–219

    Article  CAS  Google Scholar 

  • Lopez-Malo A, Alzamora SM, Argaiz A (1997) Effect of vanillin concentration, pH and incubation temperature on Aspergilllus flavus, A. niger, A. ochraceous, and A. parasiticus growth. Food Microbiol 14:117–124

    Article  CAS  Google Scholar 

  • Lopez-Malo A, Alzamora SM, Palou E (2005) Aspergillus flavus growth in the presence of chemical preservatives and naturally occurring antimicrobial compounds. Int J Food Microbiol 99:119–128

    Article  CAS  Google Scholar 

  • Lund BM, George SM, Franklin JG (1987) Inhibition of type A and type B (proteolytic) Clostridium botulinum by sorbic acid. Appl Environ Microbiol 59:935–941

    Google Scholar 

  • Mahmoud ALE (1994) Antifungal action and anti aflatoxigenic properties of some essential oil constituents. Let Appl Microbiol 19:110–113

    Article  CAS  Google Scholar 

  • Montgomery DC (1984) Design and Analysis of Experiments. John Wiley, New York

    Google Scholar 

  • Nychas GJE (1995) Natural antimicrobials from plants. In: Gould GW (ed) New Methods of Food Preservation. Blackie Academics and Professional, Glasgow, pp 58–89

    Chapter  Google Scholar 

  • Paster N, Juven BJ, Shaaya E, Menassherov M, Nitzan R, Weisslowicz H, Ravid U (1990) Inhibitory effect of oregano and thyme essential oils on moulds and food borne bacteria. Let Appl Microbiol 11:33–37

    Article  Google Scholar 

  • Paster N, Menasherov M, Ravid U, Juven B (1994) Antifungal activity of oregano and thyme essential oils applied as fumigants against fungi attacking stored grain. J Food Prot 58:81–85

    Google Scholar 

  • Skirdal IM, Eklund T (1993) Microculture model studies on the effect of sorbic acid on Penicillium chrysogenum, Cladosporium cladosporioides and Ulocladium atrum at different pH levels. J Appl Bacteriol 74:191–195

    Article  CAS  Google Scholar 

  • Sofos JN (1989) Sorbate Food Preservatives. CRC Press, Boca Raton

    Google Scholar 

  • Sofos JN, Busta FF (1980) Alternatives to the use of nitrite as an antibotulinal agent. Food Technol 34:244–251

    CAS  Google Scholar 

  • Sofos JN, Busta FF (1981) Antimicrobial activity of sorbate. J Food Prot 44:614–622

    CAS  Google Scholar 

  • Statham JA, McMeekin TA (1988) The effect of potassium sorbate on the structural integrity of Alteromonas putrefaciens. J Appl Bacteriol 65:469–476

    Article  CAS  Google Scholar 

  • Steels H, James SA, Roberts IN, Stratford M (2000) Sorbic acid resistance: the inoculum effect. Yeast 16:1173–1183

    Article  CAS  Google Scholar 

  • Thompson DP (1990) Influence of pH on the fungitoxic activity of naturally occurring compounds. J Food Prot 53:482–429

    Google Scholar 

  • Wilkins KM, Board RG (1989) Natural antimicrobial system. In: Gould GW (ed) Mechanism of Action of Food Preservation Procedures. Elsevier, New York, pp 285–362

    Google Scholar 

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Acknowledgement

The authors wish to thank the Director, CFTRI, Mysore for providing necessary facilities during the course of this investigation. We also thank Dr. R. Ravi, for analysing the statistical data.

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  1. Food Protectants and Infestation Control Department, Central Food Technological Research Institute (CSIR), Mysore, 570 020, India

    Prathesha Pillai & K. Ramaswamy

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  1. Prathesha Pillai
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  2. K. Ramaswamy
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Correspondence to K. Ramaswamy.

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Pillai, P., Ramaswamy, K. Effect of naturally occurring antimicrobials and chemical preservatives on the growth of Aspergillus Parasiticus . J Food Sci Technol 49, 228–233 (2012). https://doi.org/10.1007/s13197-011-0275-6

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  • DOI: https://doi.org/10.1007/s13197-011-0275-6

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