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Evaluation of antioxidant capacity of 13 plant extracts by three different methods: cluster analyses applied for selection of the natural extracts with higher antioxidant capacity to replace synthetic antioxidant in lamb burgers

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Abstract

The aims of this study were: to evaluate the total equivalent antioxidant capacities (TEAC) and phenolic contents of 13 plants extracts; to select the most promising extracts regarding reducing activity using cluster analysis multivariate statistical technique; and to analyse evaluate sensory acceptance of lamb burgers produced with the most promising natural antioxidants replacing sodium erythorbate. Plant extracts were evaluated regarding TEAC by DPPH• and FRAP methods, and total phenolics contents by Folin-Ciocalteau assay. The TEAC values ranged from 0.50 to 9.06 g trolox/100 g dry weight (dw) and from 43.6 to 472.32 μmol trolox/g dw for DPPH• and FRAP methods, respectively, and the total phenolic contents from 5.98 to 74.01 mg GAE/g dw. Extracts from Origanum vulgare, Melissa officinalis, Origanum majorana L. and Rosmarinus officinalis were grouped as the ones with higher antioxidant capacities by cluster analysis. All burgers produced with each one of these four plant extracts or with sodium erythorbate showed no differences (P > 0.05) regarding consumers’ sensory acceptance. In conclusion, it is possible to replace sodium erythorbate in lamb burgers by any of the four natural extracts selected without compromising sensory acceptance of this meat product.

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Acknowledgments

This study was supported by São Paulo Research Foundation - FAPESP (Case n. 2011/08093-2) and National Council for Scientific and Technological Development - CNPq (Case n. 475274/2011-3).

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Authors and Affiliations

  1. Department of Basic Sciences, College of Animal Science and Food Engineering, University of São Paulo (USP), Avenida Duque de Caxias Norte, 225, Jardim Elite, 13.635-900, Pirassununga, São Paulo, Brazil

    R. P. P. Fernandes, C. G. Lima, S. M. P. Pugine & M. P. de Melo

  2. Department of Food Engineering, College of Animal Science and Food Engineering, University of São Paulo (USP), Avenida Duque de Caxias Norte, 225, Jardim Elite, 13.635-900, Pirassununga, São Paulo, Brazil

    M. A. Trindade & P. E. S. Munekata

  3. Department of Biosystems Engineering, College of Animal Science and Food Engineering, University of São Paulo (USP), Avenida Duque de Caxias Norte, 225, Jardim Elite, 13.635-900, Pirassununga, São Paulo, Brazil

    F. G. Tonin

  4. Centro Tecnológico de la Carne de Galicia, Rúa Galicia N° 4, Parque Tecnológico de Galicia, San Cibrán das Viñas, 32900, Ourense, Spain

    J. M. Lorenzo

Authors
  1. R. P. P. Fernandes
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  2. M. A. Trindade
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  3. F. G. Tonin
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  4. C. G. Lima
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  5. S. M. P. Pugine
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  6. P. E. S. Munekata
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  7. J. M. Lorenzo
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  8. M. P. de Melo
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Corresponding author

Correspondence to R. P. P. Fernandes.

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Research highlights

• Antioxidant potential of 13 herbs through of Folin-Ciocalteau, FRAP and DPPH∙

• Oregano, marjoram, lemon balm and rosemary had higher antioxidant activity

• Cluster analysis grouped the herbs, according to results of capacity antioxidant

• Concentration of extract determined through equivalence to sodium erythorbate

• Use of natural antioxidants without compromising sensory acceptance of lamb burgers

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Fernandes, R.P.P., Trindade, M.A., Tonin, F.G. et al. Evaluation of antioxidant capacity of 13 plant extracts by three different methods: cluster analyses applied for selection of the natural extracts with higher antioxidant capacity to replace synthetic antioxidant in lamb burgers. J Food Sci Technol 53, 451–460 (2016). https://doi.org/10.1007/s13197-015-1994-x

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  • DOI: https://doi.org/10.1007/s13197-015-1994-x

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