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Antioxidant properties and phenolic profile characterization by LC–MS/MS of selected Tunisian pomegranate peels

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Abstract

Antioxidant contents and activities of different extracts from four Tunisian pomegranate peels, locally called “Acide”, “Gabsi”, “Nebli” and “Tounsi”, were studied. Peels samples were extracted with three solvents (water, ethanol and acetone). For each extract, the total phenol contents and antioxidant activity were evaluated. The highest values of polyphenol, tannins, flavonoids and anthocyanins were recorded in the acetone extract of Acide ecotype with 304.6 mg gallic acid equivalent/g; 292.23 mg gallic acid equivalent/g; 15.46 mg Quercetin/g and 54.51 mg cy-3-glu/100 g, respectively. The acetone extract of Acide ecotype also showed the highest free radical-scavenging and reducing power activity compared to other extracts. Besides, the phytochemical analysis by LC–MS/MS revealed a high content of ellagitannins with punicalagin and punicalagin derivatives as the major compounds that might be responsible for promising antioxidant activity of pomegranate peel extracts. Two compounds (Castalagin derivative and Galloyl-bis-HHDP-hex derivative) were detected only in “Acide” ecotype in important contents.

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Acknowledgement

The authors would like to thank the “Ministère de l’Enseignement Supérieur et de la Recherche Scientifique, Tunisia” for the support of this research work. They also wish to express their gratitude to Mrs. Leila MAHFOUDHI, an English teacher at the Sfax Faculty of Science, for having proofread this paper.

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Authors and Affiliations

  1. Food Analysis Laboratory, National Engineering School of Sfax (ENIS), University of Tunisia, BP 3038, Sfax, Tunisia

    Mouna Abid, Héla Yaich, Salma Cheikhrouhou, Ibtihel Khemakhem, Hamadi Attia & M. A. Ayadi

  2. Laboratory of Electrochemistry and Environnement, ENIS, Université de Sfax, BP ‘1175’, 3038, Sfax, Tunisia

    Mohamed Bouaziz

Authors
  1. Mouna Abid
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  2. Héla Yaich
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  3. Salma Cheikhrouhou
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  4. Ibtihel Khemakhem
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  5. Mohamed Bouaziz
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  6. Hamadi Attia
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  7. M. A. Ayadi
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Correspondence to M. A. Ayadi.

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Abid, M., Yaich, H., Cheikhrouhou, S. et al. Antioxidant properties and phenolic profile characterization by LC–MS/MS of selected Tunisian pomegranate peels. J Food Sci Technol 54, 2890–2901 (2017). https://doi.org/10.1007/s13197-017-2727-0

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  • DOI: https://doi.org/10.1007/s13197-017-2727-0

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