Abstract
This study was performed to evaluate the possibility of synthesizing AgNPs by aqueous extract of pre-formulated Liv-Pro-08 polyherbal formulation and characterize the synthesized AgNPs. Moreover, their antioxidant potential, cytotoxicity and hepatoprotective activity using HePG2 cell line, and performed acute and sub-acute toxicity study on male wistar rats. The aqueous extract of Liv-Pro-08 reduces the AgNO3 into AgNPs. It is primarily identified by the brown color formation in the reaction mix, and the predominant peak was found at 485 nm UV–visible spectrophotometer. The FT-IR analysis results showed that the synthesized phytomolecules capped AgNPs retain four significant functional group peaks correspond to various groups (aromatic amine, alkyne, etc.) of AgNPs. The SEM analysis states that the synthesized AgNPs were in spherical and cubic with 50–70 nm sized. As the Liv-Pro-08 contains significant antioxidant phytochemicals, it showed reasonable antioxidant and reduced power activity with the IC50 values of 711.00 µg mL and 613.75 µg mL correspondingly. The synthesized AgNPs showed an absence of cytotoxicity and possess significant hepatoprotective activity in hepatotoxin (CCl4) exposed HePG2 cell line. The acute and sub-acute toxicity of synthesized AgNPs were studied in male wistar rats. The attained results showed the absence of acute toxicity. Surprisingly, in a sub-acute study, the biomolecule contents were increased in group IV (100 mg kg body weight) treatment than control (untreated). These results suggest that the Liv-Pro-08 synthesized and phytomolecules impregnated AgNPs might be considered for drug delivery-related processes and used as a cure for liver diseases.
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The authors would like to thank the Department of Biochemistry, Periyar University, Salem for providing sufficient laboratory support and first author thank the Periyar University for providing University Research Fellowship for the successful completion of this research.
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Eswaran, A., Muthukrishnan, S., Mathaiyan, M. et al. Green synthesis, characterization and hepatoprotective activity of silver nanoparticles synthesized from pre-formulated Liv-Pro-08 poly-herbal formulation. Appl Nanosci 13, 2315–2327 (2023). https://doi.org/10.1007/s13204-021-01945-x
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DOI: https://doi.org/10.1007/s13204-021-01945-x