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UPLC–MS–QTOF analysis and antifungal activity of Cumaru (Amburana cearensis)

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Abstract

This study was aimed at investigating the phytochemical constituents, antifungal properties and antibiotic-modifying activity of the aqueous crude extract and fractions of Amburana cearensis seeds (CEFAC). The CEFAC were chemically characterized by LC–MS/MS–QTOF. In addition, the antifungal activity was assayed by the microdilution method against strains of Candida albicans. The phytochemical profile of CEFAC exhibited phenolic compounds, organic acids, and polyphenols. The results of the assessment of antifungal activity reveled an IC50 ranging from 45.6 to 2048 µg/mL. Interestingly, when CEFAC was associated with Fluconazole, we evidenced a decreased IC50 (1.81–11.9 µg/mL), suggesting a synergism with antibiotic. It was possible to identify in the crude extract and fractions several phenolic compounds, organic acids, and some polyphenols in positive ionization mode. These results suggest that CEFAC may present compounds with the ability to interact and act synergistically with antimicrobial drugs, highlighting its potential as an alternative source for the development of new antimicrobial agents.

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Acknowledgements

The autthors are grateful to the Brazilian research agencies FUNCAP, CAPES, CNPq and FINEP for the support of this work.

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Authors and Affiliations

  1. Laboratory of Chemistry and Function of Bioactive Proteins, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil

    Maria Tatiana Alves Oliveira, Geovanna Maria Medeiros Moura, Joelton Igor Oliveira da Cruz, Elizeu Antunes dos Santos & Adriana Ferreira Uchoa

  2. Department of Biomedical Science, University Centre INTA-UNINTA, Sobral, Ceará, Brazil

    Maria Tatiana Alves Oliveira, Marcus Vinícius Oliveira Barros de Alencar & Vicente de Paulo dos Anjos Landim

  3. Laboratory of Microbiology and Molecular Biology-LMBM, Regional University of Cariri-URCA, Rua Cel. Antonio Luis 1161, Pimenta, Crato, Ceará, 63105-000, Brazil

    Henrique Douglas Melo Coutinho

  4. Laboratory of Bioassays-LABIO, Educator Training Institute, Federal University of Cariri-UFCA, Campus Brejo Santo, Juazeiro do Norte, Ceará, Brazil

    Jacqueline Cosmo Andrade

  5. Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazil

    Irwin Rose Alencar de Menezes

  6. Multiuser Laboratory of Natural Products Chemistry-LMQPN, Embrapa Agroindustria Tropical, Fortaleza, Ceará, Brazil

    Paulo Riceli Vasconcelos Ribeiro & Edy Sousa de Brito

  7. Laboratory of Food Physicochemical Analysis-LAFIQ, Technology College of Cariri-FATEC, Juazeiro do Norte, Ceará, Brazil

    Erlânio Oliveira de Sousa

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  1. Maria Tatiana Alves Oliveira
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  2. Marcus Vinícius Oliveira Barros de Alencar
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  3. Vicente de Paulo dos Anjos Landim
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  6. Elizeu Antunes dos Santos
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  7. Henrique Douglas Melo Coutinho
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  8. Jacqueline Cosmo Andrade
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  13. Adriana Ferreira Uchoa
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Contributions

Methodology—antimicrobial assays (MTAO; MVOBA and GMMM); methodology—chemical analysis (PRVR; ESB and EOS); methodology—statistical analysis (IRAM; JCA and EAS); supervision o f work—(HDMC and AFU); resources (VPAL and JIOC).

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Correspondence to Henrique Douglas Melo Coutinho.

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Oliveira, M.T.A., de Alencar, M.V.O.B., de Paulo dos Anjos Landim, V. et al. UPLC–MS–QTOF analysis and antifungal activity of Cumaru (Amburana cearensis). 3 Biotech 10, 545 (2020). https://doi.org/10.1007/s13205-020-02551-4

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