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Identification of acetic acid bacteria isolated in Thailand and assigned to the genus Acetobacter by groEL gene sequence analysis

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Abstract

Twenty-three strains of acetic acid bacteria were isolated by an enrichment culture approach from fruits, flowers, mushrooms, and fermented rice products collected in Thailand. All were assigned to the genus Acetobacter and examined taxonomically for 16S rRNA gene and groEL gene sequences. Based on analysis of 16S rRNA gene and groEL gene sequences, the strains isolated were divided into ten groups: Group 1 comprised four isolates identified as A. tropicalis, Group 2 consisted of three isolates identified as A. indonesiensis, Group 3 had two isolates identified as A. persici, Group 4 was two isolates (A. orientalis), Group 5 was one isolate (A. cibinongensis), Group 6 contained three isolates (A. pasteurianus), Group 7 was one isolate of A. papayae, Group 8 was two isolates identified as A. fabarum, Group 9 was one isolate of A. okinawensis, and Group 10 was four isolates identified as A. ghanensis.

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Acknowledgments

This work was supported financially by the Strategic Scholarship/Fellowships Research Network from the Office of the Higher Education Commission, Ministry of Education (Grant no. 82/2549). Sincere thanks are also due to Mr. Richard James Goldrick, Department of Foreign Languages, Kasetsart University for English editing of this manuscript. A part of this work was carried out through collaboration in the Core to Core Program supported by the Japan Society for the Promotion of Science (JSPS) and the National Research Council of Thailand (NRCT).

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Authors and Affiliations

  1. Interdisciplinary Graduate Program in Genetic Engineering, The Graduate School, Kasetsart University, Ladyaow, Chatuchak, Bangkok, 10900, Thailand

    Nittaya Pitiwittayakul & Gunjana Theeragool

  2. BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Klong Luang, Pathum Thani, 12120, Thailand

    Pattaraporn Yukphan & Yuzo Yamada

  3. Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Ladyaow, Chatuchak, Bangkok, 10900, Thailand

    Wilawan Sintuprapa

  4. Japan International Cooperation Agency, Shibuya-ku, Tokyo, 151-8558, Japan

    Yuzo Yamada

  5. Shizuoka University, Suruga-ku, Shizuoka, 422-8529, Japan

    Yuzo Yamada

  6. Department of Microbiology, Faculty of Science, Kasetsart University, Ladyaow, Chatuchak, Bangkok, 10900, Thailand

    Gunjana Theeragool

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  1. Nittaya Pitiwittayakul
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  2. Pattaraporn Yukphan
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  3. Wilawan Sintuprapa
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  4. Yuzo Yamada
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  5. Gunjana Theeragool
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Correspondence to Gunjana Theeragool.

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Yuzo Yamada is a JICA Senior Overseas Volunteer

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Pitiwittayakul, N., Yukphan, P., Sintuprapa, W. et al. Identification of acetic acid bacteria isolated in Thailand and assigned to the genus Acetobacter by groEL gene sequence analysis. Ann Microbiol 65, 1557–1564 (2015). https://doi.org/10.1007/s13213-014-0994-9

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  • DOI: https://doi.org/10.1007/s13213-014-0994-9

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