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Chondrocyte 3D-culture in RGD-modified crosslinked hydrogel with temperature-controllable modulus

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Abstract

Based on the thermosensitivity of Pluronic® F127 and the cytointeractability of Arg-Gly-Asp (RGD) sequences, RGD-modified F127 dimethacrylate (FM-RGD) hydrogel was investigated as a 3-dimensional culture matrix for chondrocytes. Chondrocytes were encapsulated in the hydrogel by radical copolymerization of FM-RGD and FM in the presence of cells. The FM-RGD hydrogel modulus could be modulated by temperature variation from 15 °C (1,300 Pa) to the culture temperature of 37 °C (8,900 Pa), at which the hydrogel provided a condrocyte-compatible microenvironment. Compared with the hydrogel prepared from F127 dimethacrylate (FM) without RGD, the RGD-modified hydrogel produced significant (p<0.01) improvements in cell proliferation, DNA production, and viability while allowing the chondrocytes to maintain their original spherical phenotypes.

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Authors and Affiliations

  1. Center for Biomaterials, Korea Institute of Science and Technology, Seoul, 130-650, Korea

    Hyesun Lee, Jiyeon Choi, Kwideok Park & Dong Keun Han

  2. Department of Bioinspired Science (WCU), Department of Chemistry and Nano Science, Ewha Womans University, Seoul, 120-750, Korea

    Hyesun Lee, Bo Gyu Choi, Hyo Jung Moon & Byeongmoon Jeong

Authors
  1. Hyesun Lee
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  2. Bo Gyu Choi
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  3. Hyo Jung Moon
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  4. Jiyeon Choi
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  5. Kwideok Park
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  6. Byeongmoon Jeong
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  7. Dong Keun Han
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Correspondence to Byeongmoon Jeong or Dong Keun Han.

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Lee, H., Choi, B.G., Moon, H.J. et al. Chondrocyte 3D-culture in RGD-modified crosslinked hydrogel with temperature-controllable modulus. Macromol. Res. 20, 106–111 (2012). https://doi.org/10.1007/s13233-012-0074-6

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  • DOI: https://doi.org/10.1007/s13233-012-0074-6

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