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Expression and function analysis of a rice OsHSP40 gene under salt stress

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Abstract

Heat shock proteins (HSPs) play essential roles in both plant growth and abiotic stress tolerance. In rice, OsHSP40 was recently reported to regulate programmed cell death (PCD) of suspension cells under high temperature. However, the expression and functions of OsHSP40 under normal growth or other abiotic stress conditions is still unknown. We reported the expression and function of a rice OsHSP40 gene under salt stress. Homologous proteins of OsHSP40 were collected from the NCBI database and constructed the neighbor-joining (NJ) phylogenetic tree. The expression pattern of OsHSP40 was detected by qRT-PCR under NaCl (150 mM) treatment. Then, identified a rice T-DNA insertion mutant oshsp40. At last, we compared and analyzed the phenotypes of oshsp40 and wild type under salt stress. OsHSP40 was a constitutively expressed small HSP (sHSP) gene and was close related to other plant sHSPs. Moreover, the expression of OsHSP40 was regulated by salt, varying across time points and tissues. Furthermore, the growth of T-DNA insertion mutant of OsHSP40 (designated as oshsp40) was suppressed by NaCl (150 mM) compared with that of the WT at seedling stage. Detailed measurement showed root and shoot length of the oshsp40 seedlings were significantly shorter than those of the WT seedlings under NaCl stress. In addition, the pot experiment results revealed that seedlings of oshsp40 withered more seriously compared with those of WT after NaCl treatment and recovery, and that survival rate and fresh weight of oshsp40 seedlings were significantly reduced. Taken together, these data suggested that OsHSP40 had multiple functions in rice normal growth and abiotic stress tolerance.

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Acknowledgements

This research was supported by grants from the National Natural Science Foundation of China (Nos. 31760080, 31460279, 31560383 and 31660296).

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Authors and Affiliations

  1. Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, School of Life Sciences, Nanchang University, Nanchang, 330031, China

    Xin Wang, Huan Zhang, Lu-Yuan Shao, Xin Yan, Jie-Xiu Ouyang & Shao-Bo Li

  2. Medical Laboratory Education Center, Nanchang University, Nanchang, 330031, China

    Jie-Xiu Ouyang

  3. College of Life Sciences, Guangxi Normal University, Guilin, 541004, China

    Hui Peng

  4. Hunan Hi-Tech Bio-Agro Co., Ltd, Yueyang, 414400, China

    Hui Peng

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  1. Xin Wang
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  2. Huan Zhang
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  4. Xin Yan
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  5. Hui Peng
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Contributions

XW, JO and SL designed research; HZ and LS performed research; HP and XY did bioinformatic analysis and revised the manuscript; XW, JO and SL wrote the paper. All authors read and approved the final manuscript.

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Correspondence to Jie-Xiu Ouyang or Shao-Bo Li.

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Fig. S1 Expression profile of

OsHSP40in different tissues of ZH11 based on microarray analysis. The scores are the average expression values obtained from microarrays. (TIF 223 KB)

Supplementary material 2 (DOCX 18 KB)

Supplementary material 3 (DOCX 11 KB)

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Wang, X., Zhang, H., Shao, LY. et al. Expression and function analysis of a rice OsHSP40 gene under salt stress. Genes Genom 41, 175–182 (2019). https://doi.org/10.1007/s13258-018-0749-2

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  • DOI: https://doi.org/10.1007/s13258-018-0749-2

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